Biodegradation of chloroaromatic compounds by bacteria isolated from as-Samra wastewater treatment plant
Dissertant
Thesis advisor
Irshaid, Fawzi
al-Tarawinah, Khalid Ahmad
Comitee Members
Jaran, Adnan S.
Khalaf, Maruf A.
Khulayfat, Khalid Muhammad
University
Al albayt University
Faculty
Faculty of Sciences
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2009
English Abstract
Citrobacter fundi was isolated from the As-Samra wastewater treatment plant, which is located in northern Jordan.
It was identified by using morphological and biochemical characteristics.
Citrobacter fundi were found to use the chlorobenzoate compounds as alternative carbon and energy source.
The percentages of degradation of these chlorobenzoate (2- chloro benzoic acid, 3- chloro benzoic acid, 4- chloro benzoic acid and 3,4- di chloro benzoic acid) at concentration 2-mM were found 70.4 %, 39.5 %, 38% and 61 % respectively, after 80 h of incubation.
At 3mM concentration the rates were 82%, 32 %, 49 % and 44.5 % respectively.
The initial rates were found different according to the substrate and its concentration.
The highest initial rates were obtained with the 3mM concentration for 2CBA.
The order of initial rates was 64.3µM / h (2- chloro benzoic acid), 50.7 µM / h (4- chloro benzoic acid), 42.2 µM / h (3, 4- di chloro benzoic acid) and 6.2 µM / h (3- chloro benzoic acid).
The biodegradation ability was monitored through the release of chloride, disappearance of the substrate and finally the growth of bacterial cells on that substrate.
The best results of degradation of 2-CBA compound were obtained with 3mM substrate concentration, 37ºC, pH 7and 100µl inoculums size.
Adaptation of the cells on 2-CBA for 48 hours and cells starvation for 24 hours and 48 hours increased slightly the initial degradation rate.
The carbon sources increased the cell biomass.
However, all carbon sources used reduced the biodegradation and Cl production from 2-CBA.
All glucose concentration enhanced the growth and reduced the degradation percentage as well as the Cl production.
Yeast extract and casein reduced the degradation activity of the 2-CBA as well as the chlorine release from it.
2-CBA (3mM) was used in further experiments because the 2-CBA is the most toxic one among these compounds.
The experiments included substrate concentration, temperature, pH, inoculum size, carbon starvation, carbon adaptation, and carbon and nitrogen sources.
The preferential concentration, temperature and pH for the strain were 3mM, 37ºC and 7 respectively.
Main Subjects
Earth Sciences, Water and Environment
Topics
No. of Pages
104
Table of Contents
Table of contents.
Abstract.
Introduction.
Chapter One : literature review.
Chapter Two : design and methodology.
Chapter Three : results.
Chapter Four : discussions.
References
References.
American Psychological Association (APA)
al-Rgibat, Iman Ahmad. (2009). Biodegradation of chloroaromatic compounds by bacteria isolated from as-Samra wastewater treatment plant. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-307526
Modern Language Association (MLA)
al-Rgibat, Iman Ahmad. Biodegradation of chloroaromatic compounds by bacteria isolated from as-Samra wastewater treatment plant. (Master's theses Theses and Dissertations Master). Al albayt University. (2009).
https://search.emarefa.net/detail/BIM-307526
American Medical Association (AMA)
al-Rgibat, Iman Ahmad. (2009). Biodegradation of chloroaromatic compounds by bacteria isolated from as-Samra wastewater treatment plant. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-307526
Language
English
Data Type
Arab Theses
Record ID
BIM-307526
