Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan

العناوين الأخرى

تأثير المضادات البكتيرية و مضادات الأكسدة لبعض مستخلصات النباتات التي تنمو في شمال الأردن

مقدم أطروحة جامعية

Izz al-Arab, Majidah Ali Hamad

مشرف أطروحة جامعية

Jaran, Adnan S.
al-Tarawinah, Khalid Ahmad

أعضاء اللجنة

Khulayfat, Khalid Muhammad
Mahasinah, Ihsan
Irshid, Fawzi

الجامعة

جامعة آل البيت

الكلية

كلية العلوم

القسم الأكاديمي

قسم العلوم الحياتية

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2009

الملخص الإنجليزي

The activities of methanolic (MeOH) extracts of four plants : Achillea fragrantissima (forssk) (A.

fragrantissima), Teucrium polium L.

(T.

polium), Rosmarinus officinalis and Alhgi graecorum were investigated in this study, as antibacterial and antioxidant.

The effect of methanolic plant extracts was tested against five bacterial isolates: Escherichia coli (E.

coli ATCC 25922), Staphylococcus aureus (S.

aureus ATCC 25923), Pseudomonas aeruginosa (P.

aeruginosa ATCC 27853), Proteus mirabills ATCC 426 and Enterobacter cloacae ATCC 29004.

The plant extracts have different effects on these bacterial strains.

The inhibition zones of these plant extracts ranged from 0-25mm.

The antibacterial activity against Gram negative bacteria was noticed with the four plants extract.

However, the effect on gram positive was also noticed with the Rosmarinus officinalis plant at lower concentration(10mg / ml).

The activity increases as the concentrations of plant extracts increase.

The minimum inhibitory concentration (MIC) for MeOH plant extracts was found between 1.2-3 mg / ml for A.

fragrantissima, 1.2- 1.8 mg / ml for Alhgi graecorum, 1.2-2.6 for T.

polium and 1.2-1.7 for Rosmarinus officinalis against all bacterial strains.

A highest antioxidant activity was obtained with extract of Rosmarinus officinalis (97.9% inhibition of ABTS•+) and the lowest antioxidant was obtained with Alhgi graecorum (38.9 % inhibition of ABTS•+).

The IC50 for the four plants used ranged from 20.8µg / ml for Rosmarinus officinalis and 103.04µg / ml for A.

fragrantissima.

The trolox equivalent antioxidant capacity of R.

officinalis, A.

fragrantissima, T.

polium and A.

graecorum was found 0.424, 0.192, 0.174, 0.172 µM/µg dry matter respectively.

The IC50 in trolox equivalent antioxidant capacity for these plants was 0.53 µM / µg for R.

officinalis, 0.011 µM / µg for A.

fragrantissima, 0.158 µM / µg for T.

polium and 0.165µM / µg for A.

graecorum..

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

62

قائمة المحتويات

Table of contents.

Abstract.

Introduction.

Chapter One : Literature review.

Chapter Two : Material and methods.

Chapter Three : Results.

Chapter Four : Discussion, conclusion, recommendation.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Izz al-Arab, Majidah Ali Hamad. (2009). Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-314831

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Izz al-Arab, Majidah Ali Hamad. Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University. (2009).
https://search.emarefa.net/detail/BIM-314831

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Izz al-Arab, Majidah Ali Hamad. (2009). Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-314831

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-314831