Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan
Other Title(s)
تأثير المضادات البكتيرية و مضادات الأكسدة لبعض مستخلصات النباتات التي تنمو في شمال الأردن
Dissertant
Izz al-Arab, Majidah Ali Hamad
Thesis advisor
Jaran, Adnan S.
al-Tarawinah, Khalid Ahmad
Comitee Members
Khulayfat, Khalid Muhammad
Mahasinah, Ihsan
Irshid, Fawzi
University
Al albayt University
Faculty
Faculty of Sciences
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2009
English Abstract
The activities of methanolic (MeOH) extracts of four plants : Achillea fragrantissima (forssk) (A.
fragrantissima), Teucrium polium L.
(T.
polium), Rosmarinus officinalis and Alhgi graecorum were investigated in this study, as antibacterial and antioxidant.
The effect of methanolic plant extracts was tested against five bacterial isolates: Escherichia coli (E.
coli ATCC 25922), Staphylococcus aureus (S.
aureus ATCC 25923), Pseudomonas aeruginosa (P.
aeruginosa ATCC 27853), Proteus mirabills ATCC 426 and Enterobacter cloacae ATCC 29004.
The plant extracts have different effects on these bacterial strains.
The inhibition zones of these plant extracts ranged from 0-25mm.
The antibacterial activity against Gram negative bacteria was noticed with the four plants extract.
However, the effect on gram positive was also noticed with the Rosmarinus officinalis plant at lower concentration(10mg / ml).
The activity increases as the concentrations of plant extracts increase.
The minimum inhibitory concentration (MIC) for MeOH plant extracts was found between 1.2-3 mg / ml for A.
fragrantissima, 1.2- 1.8 mg / ml for Alhgi graecorum, 1.2-2.6 for T.
polium and 1.2-1.7 for Rosmarinus officinalis against all bacterial strains.
A highest antioxidant activity was obtained with extract of Rosmarinus officinalis (97.9% inhibition of ABTS•+) and the lowest antioxidant was obtained with Alhgi graecorum (38.9 % inhibition of ABTS•+).
The IC50 for the four plants used ranged from 20.8µg / ml for Rosmarinus officinalis and 103.04µg / ml for A.
fragrantissima.
The trolox equivalent antioxidant capacity of R.
officinalis, A.
fragrantissima, T.
polium and A.
graecorum was found 0.424, 0.192, 0.174, 0.172 µM/µg dry matter respectively.
The IC50 in trolox equivalent antioxidant capacity for these plants was 0.53 µM / µg for R.
officinalis, 0.011 µM / µg for A.
fragrantissima, 0.158 µM / µg for T.
polium and 0.165µM / µg for A.
graecorum..
Main Subjects
Topics
No. of Pages
62
Table of Contents
Table of contents.
Abstract.
Introduction.
Chapter One : Literature review.
Chapter Two : Material and methods.
Chapter Three : Results.
Chapter Four : Discussion, conclusion, recommendation.
References.
American Psychological Association (APA)
Izz al-Arab, Majidah Ali Hamad. (2009). Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-314831
Modern Language Association (MLA)
Izz al-Arab, Majidah Ali Hamad. Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University. (2009).
https://search.emarefa.net/detail/BIM-314831
American Medical Association (AMA)
Izz al-Arab, Majidah Ali Hamad. (2009). Antibacterial and antioxidant activity of methanolic extracts of some plant selected from northern Badia region-Jordan. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-314831
Language
English
Data Type
Arab Theses
Record ID
BIM-314831
