In vitro and in vivo studies of secondary metabolites extracted from some algae against protoscolices of echinococcus granulosus in laboratory mice balb C strain in comparison toalbendazole activity

العناوين الأخرى

دراسات خارج و داخل الجسم الحي لمركبات الأيض الثانوي المستخلصة من بعض الطحالب ضد الرؤيسات الأولية لطفيلي المشوكة الحبيبية في الفئران المختبرية سلالة balb C مقارنة بفعالية الألبندازول

مقدم أطروحة جامعية

Khalaf, Amal Khudayr

مشرف أطروحة جامعية

Adhbi, Ahmad Muhsin
al-Mayyah, Subayh Hulayyil

الجامعة

جامعة البصرة

الكلية

كلية التربية العلمية

القسم الأكاديمي

قسم علوم الحياة

دولة الجامعة

العراق

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2012

الملخص الإنجليزي

-The present study aims at testing the bioactivity of the chemical compounds extracted from two species of algae Cladophora crispata (Chlorophyta ) and Hapalosiphon aureus (Cyanophyta) against hydatid disease caused by the parasite Echinococcus granulosus and compared their activity with that of albendazole drug which used to treat the hydatid disease.

The extracted bioactive compounds were identified by using GC- Mass technique .

The present study involved two main aspects: The first aspect: In vitro activity involves the study of the activity of the compounds on protoscolices viability compared with albendazole , where it was found that the compounds of the ethylacetate extract of C.

crispata have more activity than other extracts and equal to the activity of albendazole , also the present results revealed found that the protoscolices treated with ethylacetate extract are killed at fifth day – post treatment in concentrations 110 and 120 μg / ml whereas the protoscolices treated with methanol extract are killed in fifth day but only in one concentration .

The hexane extract of the same algae has recorded a positive activity against the protoscolices at fifth day – post treatment but the activity is record at sixth day – post treatment of H.

aureus .

Alkaloid compounds scores a lower activity than that recorded for other extracts and for the two algae where the activity is recorded at sixth day – post treatment for C.

crispata and at the seventh day for H.

aureus.

The second aspect : In vivo activity involves the activity of the ethylacetate extract and fatty acids compounds of methanol and hexane extracts against hydatid cysts of experimental animals where the results showed decreases in the number of cysts , calcification of cysts and decreases in the size of the cysts as well decreases in the weight of infected animals after treatment comparing with albendazole .

The observations of the physiological state of the groups treated with bioactive compound revealed that the number of lymphocytes were decreased whereas the number of neutrophils were increased comparing with the positive group but the number of lymphocytes increased in the groups treated with albendazole while the number of the neutrophiles decreased.

The biochemical parameters (Blood urea test, Aspartate aminotransferase, and serum glucose concentration) were also observed in the present study in both treated groups (positive and negative groups).

The statistical analysis of these parameters has proved the presence of significant differences among the studied groups .

The chemical analysis of bioactive compounds revealed that the alkaloid extract of C.

crispata contains a high percentage of Pyridine,2,3,4,5-tetrahydro compound.

Ethylacetate extract contains 2-(N,N-dimethylhydrazino) cyclohexanecarbonitrile compound , also methanol and hexane extracts of the same algae contains ester compounds of the following fatty acids (2- deca - 3,ddienyloxy) carbonyl benzeoic acid and Nonandioic acid , dimethyl ester while the alkaloid extract of H.

aureus contains 2- methyl-pyroline compound in a high percentage and its hexane extract contains the ethylhexyl phthalate compound in a high percentage too.

التخصصات الرئيسية

الأحياء

الموضوعات

• الأدوية
• الطحالب

عدد الصفحات

162

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-317116