In vitro and in vivo studies of secondary metabolites extracted from some algae against protoscolices of echinococcus granulosus in laboratory mice balb C strain in comparison toalbendazole activity
Other Title(s)
دراسات خارج و داخل الجسم الحي لمركبات الأيض الثانوي المستخلصة من بعض الطحالب ضد الرؤيسات الأولية لطفيلي المشوكة الحبيبية في الفئران المختبرية سلالة balb C مقارنة بفعالية الألبندازول
Dissertant
Thesis advisor
Adhbi, Ahmad Muhsin
al-Mayyah, Subayh Hulayyil
University
University of Basrah
Faculty
Education of Science College
Department
Department of Biology
University Country
Iraq
Degree
Ph.D.
Degree Date
2012
English Abstract
-The present study aims at testing the bioactivity of the chemical compounds extracted from two species of algae Cladophora crispata (Chlorophyta ) and Hapalosiphon aureus (Cyanophyta) against hydatid disease caused by the parasite Echinococcus granulosus and compared their activity with that of albendazole drug which used to treat the hydatid disease.
The extracted bioactive compounds were identified by using GC- Mass technique .
The present study involved two main aspects: The first aspect: In vitro activity involves the study of the activity of the compounds on protoscolices viability compared with albendazole , where it was found that the compounds of the ethylacetate extract of C.
crispata have more activity than other extracts and equal to the activity of albendazole , also the present results revealed found that the protoscolices treated with ethylacetate extract are killed at fifth day – post treatment in concentrations 110 and 120 μg / ml whereas the protoscolices treated with methanol extract are killed in fifth day but only in one concentration .
The hexane extract of the same algae has recorded a positive activity against the protoscolices at fifth day – post treatment but the activity is record at sixth day – post treatment of H.
aureus .
Alkaloid compounds scores a lower activity than that recorded for other extracts and for the two algae where the activity is recorded at sixth day – post treatment for C.
crispata and at the seventh day for H.
aureus.
The second aspect : In vivo activity involves the activity of the ethylacetate extract and fatty acids compounds of methanol and hexane extracts against hydatid cysts of experimental animals where the results showed decreases in the number of cysts , calcification of cysts and decreases in the size of the cysts as well decreases in the weight of infected animals after treatment comparing with albendazole .
The observations of the physiological state of the groups treated with bioactive compound revealed that the number of lymphocytes were decreased whereas the number of neutrophils were increased comparing with the positive group but the number of lymphocytes increased in the groups treated with albendazole while the number of the neutrophiles decreased.
The biochemical parameters (Blood urea test, Aspartate aminotransferase, and serum glucose concentration) were also observed in the present study in both treated groups (positive and negative groups).
The statistical analysis of these parameters has proved the presence of significant differences among the studied groups .
The chemical analysis of bioactive compounds revealed that the alkaloid extract of C.
crispata contains a high percentage of Pyridine,2,3,4,5-tetrahydro compound.
Ethylacetate extract contains 2-(N,N-dimethylhydrazino) cyclohexanecarbonitrile compound , also methanol and hexane extracts of the same algae contains ester compounds of the following fatty acids (2- deca - 3,ddienyloxy) carbonyl benzeoic acid and Nonandioic acid , dimethyl ester while the alkaloid extract of H.
aureus contains 2- methyl-pyroline compound in a high percentage and its hexane extract contains the ethylhexyl phthalate compound in a high percentage too.
Main Subjects
Topics
No. of Pages
162
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction and literature review.
Chapter Two : materials and methods.
Chapter Three : results.
Chapter Four : discussion.
References.
American Psychological Association (APA)
Khalaf, Amal Khudayr. (2012). In vitro and in vivo studies of secondary metabolites extracted from some algae against protoscolices of echinococcus granulosus in laboratory mice balb C strain in comparison toalbendazole activity. (Doctoral dissertations Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317116
Modern Language Association (MLA)
Khalaf, Amal Khudayr. In vitro and in vivo studies of secondary metabolites extracted from some algae against protoscolices of echinococcus granulosus in laboratory mice balb C strain in comparison toalbendazole activity. (Doctoral dissertations Theses and Dissertations Master). University of Basrah. (2012).
https://search.emarefa.net/detail/BIM-317116
American Medical Association (AMA)
Khalaf, Amal Khudayr. (2012). In vitro and in vivo studies of secondary metabolites extracted from some algae against protoscolices of echinococcus granulosus in laboratory mice balb C strain in comparison toalbendazole activity. (Doctoral dissertations Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317116
Language
English
Data Type
Arab Theses
Record ID
BIM-317116
