Production of transglutaminase by a local Streptomyces isolate using wheat bran

الملخص EN

Transglutaminase (TGase) was produced by a locally isolated Streptomyces sp.

strain using solid fermentation with wheat bran as substrate.

The highest product yield was obtained with 70 % moisture content ; 15 % inoculums size (volume per mass) and incubation period of 6 days.

Among different carbon sources, starch (4 %) resulted in maximum enzyme production followed by glycerol and glucose.

The best nitrogen source was peptone (3 %) followed by yeast extract.

The highest TGase activity was observed at 45 °C and pH 6.5 with a good activity in the range of 6-7.5.

The enzyme was stable at 40 to 45°C.

It was found to be highly stable within the range of 5-8.5 pH values.