Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran
المؤلفون المشاركون
Alizadah, R.
Hushyar, H.
Bandihpur, M.
Arbabi, M.
Kazimi, F.
Talari, A.
Kazimi, B.
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 13، العدد 12 (31 ديسمبر/كانون الأول 2011)، ص ص. 863-867، 5ص.
الناشر
تاريخ النشر
2011-12-31
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
5
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis.
This study aims to determine the drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran.
Methods : Ninety seven samples were collected from ulcers of leishmaniasis patients from some endemic areasof Iran.
The Giemsa stained samples were examined microscopically and cultured in NNN and RPMI 1640 mediums for parasite detection.
After DNA extraction, PCR was done by a pair of specific primers.
For detection of mutation in DNA, first PCR products were electrophoresed on CSGE gel.
The suspected samples were compared by sequencing and RFLP results were demonstrated.
Comparison of DNA derived from a wild type cell and mutant cell was undertaken by CSGE and sequencing methods.
Results : Among 90 isolates (92.8 %) examined for detection of mutation in gene with CSGE and RFLP, 10 (11.1 %) revealed a disorder in sequencing selection for unresponsive to drug.
Conclusion : Drug resistance in cutaneous leishmaniasis to sodium stiboglocanat is probably due to a mutation in a genome.
A field study is needed to determine the distribution of drug resistance and other gene mutations involved in unresponsiveness to drugs in leishmaniasis endemic areas of Iran.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alizadah, R.& Hushyar, H.& Bandihpur, M.& Arbabi, M.& Kazimi, F.& Talari, A.…[et al.]. 2011. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal،Vol. 13, no. 12, pp.863-867.
https://search.emarefa.net/detail/BIM-321548
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alizadah, R.…[et al.]. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal Vol. 13, no. 12 (Dec. 2011), pp.863-867.
https://search.emarefa.net/detail/BIM-321548
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alizadah, R.& Hushyar, H.& Bandihpur, M.& Arbabi, M.& Kazimi, F.& Talari, A.…[et al.]. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal. 2011. Vol. 13, no. 12, pp.863-867.
https://search.emarefa.net/detail/BIM-321548
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 866-867
رقم السجل
BIM-321548
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر