Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran
Joint Authors
Alizadah, R.
Hushyar, H.
Bandihpur, M.
Arbabi, M.
Kazimi, F.
Talari, A.
Kazimi, B.
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 13, Issue 12 (31 Dec. 2011), pp.863-867, 5 p.
Publisher
Publication Date
2011-12-31
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis.
This study aims to determine the drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran.
Methods : Ninety seven samples were collected from ulcers of leishmaniasis patients from some endemic areasof Iran.
The Giemsa stained samples were examined microscopically and cultured in NNN and RPMI 1640 mediums for parasite detection.
After DNA extraction, PCR was done by a pair of specific primers.
For detection of mutation in DNA, first PCR products were electrophoresed on CSGE gel.
The suspected samples were compared by sequencing and RFLP results were demonstrated.
Comparison of DNA derived from a wild type cell and mutant cell was undertaken by CSGE and sequencing methods.
Results : Among 90 isolates (92.8 %) examined for detection of mutation in gene with CSGE and RFLP, 10 (11.1 %) revealed a disorder in sequencing selection for unresponsive to drug.
Conclusion : Drug resistance in cutaneous leishmaniasis to sodium stiboglocanat is probably due to a mutation in a genome.
A field study is needed to determine the distribution of drug resistance and other gene mutations involved in unresponsiveness to drugs in leishmaniasis endemic areas of Iran.
American Psychological Association (APA)
Alizadah, R.& Hushyar, H.& Bandihpur, M.& Arbabi, M.& Kazimi, F.& Talari, A.…[et al.]. 2011. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal،Vol. 13, no. 12, pp.863-867.
https://search.emarefa.net/detail/BIM-321548
Modern Language Association (MLA)
Alizadah, R.…[et al.]. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal Vol. 13, no. 12 (Dec. 2011), pp.863-867.
https://search.emarefa.net/detail/BIM-321548
American Medical Association (AMA)
Alizadah, R.& Hushyar, H.& Bandihpur, M.& Arbabi, M.& Kazimi, F.& Talari, A.…[et al.]. Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran. Iranian Red Crescent Medical Journal. 2011. Vol. 13, no. 12, pp.863-867.
https://search.emarefa.net/detail/BIM-321548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 866-867
Record ID
BIM-321548