Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts
المؤلفون المشاركون
al-Saymari, Ihsan Edan
al-Shimari, Maani N.
al-Fayyd, Muhammad M. A.
المصدر
Iraqi Journal of Biotechnology
العدد
المجلد 11، العدد 2 (31 ديسمبر/كانون الأول 2012)، ص ص. 248-260، 13ص.
الناشر
جامعة بغداد معهد الهندسة الوراثية و التقنيات الإحيائية للدراسات العليا
تاريخ النشر
2012-12-31
دولة النشر
العراق
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
This research includes a study of Hydatid Cysts E.
granulosus (larval stage on the molecular level, where 7 samples of hydatid cysts were collected from parasite intermediate hosts " Human (Liver, Spleen, Lung) and liver of Sheep, Goat, Cattle and Buffaloes".
DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various periods in 70% ethanol .
Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by Polymerase Chain Reaction (delete) (PCR) to determine genetic variation depending on Random Amplified Polymorphic DNA .
In the present study 10 primers have been used, during which the genetic variations were revealed among isolated (extracted DNA) of hydatid cysts which was collected from human and other intermediate hoists except Cows and Buffaloes.
The current results of this study have shown the following :1 - It was found one primer (OPA – 01) was able to diagnose sample numbered 1 which represent the isolated DNA of liver hydatid cyst which was obtained from human at age group 10 – 20 years old.
2 - The ability of primer OPC – 10 to determine fingerprinting of DNA sample of Sheep liver hydatid cyst.
3 - The ability of primer OPC – 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 – 40 years old.
4 - The ability of primer OPE – 07 to determine fingerprinting of DNA sample of Goat liver hydatid cyst.
5– Amplification process to the DNA samples which extracted from Cows and Buffaloes liver hydatid cysts wasn’t completed by using all 10 primers.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Saymari, Ihsan Edan& al-Shimari, Maani N.& al-Fayyd, Muhammad M. A.. 2012. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology،Vol. 11, no. 2, pp.248-260.
https://search.emarefa.net/detail/BIM-322948
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Saymari, Ihsan Edan…[et al.]. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology Vol. 11, no. 2 (2012), pp.248-260.
https://search.emarefa.net/detail/BIM-322948
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Saymari, Ihsan Edan& al-Shimari, Maani N.& al-Fayyd, Muhammad M. A.. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology. 2012. Vol. 11, no. 2, pp.248-260.
https://search.emarefa.net/detail/BIM-322948
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 258-260
رقم السجل
BIM-322948
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر