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Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts
Joint Authors
al-Saymari, Ihsan Edan
al-Shimari, Maani N.
al-Fayyd, Muhammad M. A.
Source
Iraqi Journal of Biotechnology
Issue
Vol. 11, Issue 2 (31 Dec. 2012), pp.248-260, 13 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2012-12-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
This research includes a study of Hydatid Cysts E.
granulosus (larval stage on the molecular level, where 7 samples of hydatid cysts were collected from parasite intermediate hosts " Human (Liver, Spleen, Lung) and liver of Sheep, Goat, Cattle and Buffaloes".
DNA was extracted from germinal layer cells of hydatid cysts which were isolated shortly or preserved for various periods in 70% ethanol .
Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by Polymerase Chain Reaction (delete) (PCR) to determine genetic variation depending on Random Amplified Polymorphic DNA .
In the present study 10 primers have been used, during which the genetic variations were revealed among isolated (extracted DNA) of hydatid cysts which was collected from human and other intermediate hoists except Cows and Buffaloes.
The current results of this study have shown the following :1 - It was found one primer (OPA – 01) was able to diagnose sample numbered 1 which represent the isolated DNA of liver hydatid cyst which was obtained from human at age group 10 – 20 years old.
2 - The ability of primer OPC – 10 to determine fingerprinting of DNA sample of Sheep liver hydatid cyst.
3 - The ability of primer OPC – 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 – 40 years old.
4 - The ability of primer OPE – 07 to determine fingerprinting of DNA sample of Goat liver hydatid cyst.
5– Amplification process to the DNA samples which extracted from Cows and Buffaloes liver hydatid cysts wasn’t completed by using all 10 primers.
American Psychological Association (APA)
al-Saymari, Ihsan Edan& al-Shimari, Maani N.& al-Fayyd, Muhammad M. A.. 2012. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology،Vol. 11, no. 2, pp.248-260.
https://search.emarefa.net/detail/BIM-322948
Modern Language Association (MLA)
al-Saymari, Ihsan Edan…[et al.]. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology Vol. 11, no. 2 (2012), pp.248-260.
https://search.emarefa.net/detail/BIM-322948
American Medical Association (AMA)
al-Saymari, Ihsan Edan& al-Shimari, Maani N.& al-Fayyd, Muhammad M. A.. Molecular characterization of antigens extracted from hydatid cysts of human and other intermediate hosts. Iraqi Journal of Biotechnology. 2012. Vol. 11, no. 2, pp.248-260.
https://search.emarefa.net/detail/BIM-322948
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 258-260
Record ID
BIM-322948