Identification and determination of aflatoxin G1 and aflatoxigenic aspergillus isolates from dried vine fruits in duhok by LC MS-MS technique

الملخص EN

The study was undertaken to investigate the mycobiota of the 20 samples of dried vine fruits (Zibib) used for preparation of grape juice collected from local shops for soft drinks and fruit juices during August, 2010 and for natural contamination with aflatoxin G1.

Aflatoxigenic strains of Aspergillus were detected by culture based methods, these include fluorescence upon exposure to UV long wave length (365 nm) light and pigment production in coconut agar medium after exposure to ammonium hydroxide.

All tested of A.

parasiticus showed aflatoxigenic potential and the ratio of aflatoxigenic isolates of A.

flavus was higher than non aflatoxigenic strains.

Natural contamination of grape juice with afflatoxin G1 was detected by LC / MS-MS technique.

Out of 14 juice samples 7 were be found to contaminated with AFG1.