Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages
المؤلف
المصدر
Journal of the Faculty of Medicine Baghdad
العدد
المجلد 55، العدد 2 (30 يونيو/حزيران 2013)، ص ص. 182-185، 4ص.
الناشر
تاريخ النشر
2013-06-30
دولة النشر
العراق
عدد الصفحات
4
التخصصات الرئيسية
الموضوعات
الملخص EN
Background : mitochondria are the cell’s powerhouse, the site where the vast majority of ATP is synthesized.
Mitochondrial activity represents a central checkpoint for detection of the difference between cancer cells and normal cells at the metabolic profile.
Objective : to find out if there is a correlation between in vitro transformation and mitochondrial activity, by measuring the activity during clonal evolution of the locally established rat embryo fibroblast (REF) cell line throughout studying different passages.
Materials & Methods : the (REF) cell line, was in vitro cultured.
Mitochondria were isolated by differential centrifugation following Mitochondria Isolation Kit.
Enzymatic activity of intact mitochondria has been measured using Cytochrome C Oxidase Activity Assay Kit.
The decrease in absorbance at 550nm as horse heart Ferro cytochrome c was oxidized has been monitored.
Results : depending on the conducted colorimetric assay, cytochrome c oxidase activity was measured, for the three different passages (42, 72 and 91) of REF cell line.
At 550 nm, spectral data were valued (0.247, 0.723 and 1.318) unit / ml for three passages of REF cells (42, 72 and 91) respectively.
Conclusion : there was a significant relationship between the mitochondrial activity and the age (passage number) for these in vitro cultured cells.
These variations probably due to the transformational events that have been occurred during long-term continuous subculture.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kazim, Husam al-Din Muhammad. 2013. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad،Vol. 55, no. 2, pp.182-185.
https://search.emarefa.net/detail/BIM-336279
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kazim, Husam al-Din Muhammad. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad Vol. 55, no. 2 (2013), pp.182-185.
https://search.emarefa.net/detail/BIM-336279
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kazim, Husam al-Din Muhammad. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad. 2013. Vol. 55, no. 2, pp.182-185.
https://search.emarefa.net/detail/BIM-336279
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 184-185
رقم السجل
BIM-336279
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر