Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages
Author
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 55, Issue 2 (30 Jun. 2013), pp.182-185, 4 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2013-06-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
Background : mitochondria are the cell’s powerhouse, the site where the vast majority of ATP is synthesized.
Mitochondrial activity represents a central checkpoint for detection of the difference between cancer cells and normal cells at the metabolic profile.
Objective : to find out if there is a correlation between in vitro transformation and mitochondrial activity, by measuring the activity during clonal evolution of the locally established rat embryo fibroblast (REF) cell line throughout studying different passages.
Materials & Methods : the (REF) cell line, was in vitro cultured.
Mitochondria were isolated by differential centrifugation following Mitochondria Isolation Kit.
Enzymatic activity of intact mitochondria has been measured using Cytochrome C Oxidase Activity Assay Kit.
The decrease in absorbance at 550nm as horse heart Ferro cytochrome c was oxidized has been monitored.
Results : depending on the conducted colorimetric assay, cytochrome c oxidase activity was measured, for the three different passages (42, 72 and 91) of REF cell line.
At 550 nm, spectral data were valued (0.247, 0.723 and 1.318) unit / ml for three passages of REF cells (42, 72 and 91) respectively.
Conclusion : there was a significant relationship between the mitochondrial activity and the age (passage number) for these in vitro cultured cells.
These variations probably due to the transformational events that have been occurred during long-term continuous subculture.
American Psychological Association (APA)
Kazim, Husam al-Din Muhammad. 2013. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad،Vol. 55, no. 2, pp.182-185.
https://search.emarefa.net/detail/BIM-336279
Modern Language Association (MLA)
Kazim, Husam al-Din Muhammad. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad Vol. 55, no. 2 (2013), pp.182-185.
https://search.emarefa.net/detail/BIM-336279
American Medical Association (AMA)
Kazim, Husam al-Din Muhammad. Mitochondrial activity of the locally established rat embryo fibroblast cell line through different passages. Journal of the Faculty of Medicine Baghdad. 2013. Vol. 55, no. 2, pp.182-185.
https://search.emarefa.net/detail/BIM-336279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 184-185
Record ID
BIM-336279