Spectrophotometric determination of teicoplanin via coupling with diazotized p-nitroaniline

الملخص EN

A new, simple and sensitive spectrophotometric method was described for the determination of teicoplanin as a pure material and in pharmaceutical formulation.

The method is based on the coupling of teicoplanin with diazotized p-nitroaniline in alkaline medium.

The dye shows an absorption maximum at 490 nm and obeys Beer's law over a range of 2 to 80 μg.ml.-1 of teicoplanin.

The molar absorptivity of the coloured azo dye is 208 x 104l.

mol-1cm-1.

The relative standard deviation does not exceed 2.7 % (n = 5).

The azo dye formed is stable for 60 minutes.

The method was applied successfully to the assay of teicoplanin in pharmaceutical preparation (injection).