Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region

العناوين الأخرى

استخلاص المواد الحيوية من البكتيريا المعزولة من منطقة الكرك-عين سارة و دراسة تأثيرها كمضادات بكتيرية

مقدم أطروحة جامعية

al-Dirdisawi, Bakr Sakha

مشرف أطروحة جامعية

Khulayfat, Khalid Muhammad

أعضاء اللجنة

al-Zaraini, Wail Ali Hasan

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2013

الملخص الإنجليزي

Thirty three soil bacterial isolates were obtained from Al-Karak–Ain / sarah.

The crude extract of nine strains showed antibacterial activity.

They were screened for their antibacterial activity against five types of pathogenic microorganisms (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Micrococcu luteus, Enterobacter aerugenes).

One of these isolated (D4) was shown to be the most effective strain.

Bacteria strain was coded by D4 identified as Alcaligenes faecalis based on RAPID CB plus kit.

Optimization of the conditions for production of bioactive extract was performed.

The crude extract of Alcaligenes faecalis sp.

D4 has narrow antibacterial activity against Staphylococcus aureus and Micrococcu luteus.

The crude extract of bacterial of interest was subjected to partial purification by column chromatography.

The bioactivity of the partially purified compounds was tested by agar diffusion assay and their minimum inhibition concentration (MIC) was determined.

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

28

قائمة المحتويات

Table of contents.

Abstract.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results and discussion.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Dirdisawi, Bakr Sakha. (2013). Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346892

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Dirdisawi, Bakr Sakha. Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University. (2013).
https://search.emarefa.net/detail/BIM-346892

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Dirdisawi, Bakr Sakha. (2013). Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346892

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-346892