Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region
Other Title(s)
استخلاص المواد الحيوية من البكتيريا المعزولة من منطقة الكرك-عين سارة و دراسة تأثيرها كمضادات بكتيرية
Dissertant
Thesis advisor
Comitee Members
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2013
English Abstract
Thirty three soil bacterial isolates were obtained from Al-Karak–Ain / sarah.
The crude extract of nine strains showed antibacterial activity.
They were screened for their antibacterial activity against five types of pathogenic microorganisms (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Micrococcu luteus, Enterobacter aerugenes).
One of these isolated (D4) was shown to be the most effective strain.
Bacteria strain was coded by D4 identified as Alcaligenes faecalis based on RAPID CB plus kit.
Optimization of the conditions for production of bioactive extract was performed.
The crude extract of Alcaligenes faecalis sp.
D4 has narrow antibacterial activity against Staphylococcus aureus and Micrococcu luteus.
The crude extract of bacterial of interest was subjected to partial purification by column chromatography.
The bioactivity of the partially purified compounds was tested by agar diffusion assay and their minimum inhibition concentration (MIC) was determined.
Main Subjects
Topics
No. of Pages
28
Table of Contents
Table of contents.
Abstract.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
al-Dirdisawi, Bakr Sakha. (2013). Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346892
Modern Language Association (MLA)
al-Dirdisawi, Bakr Sakha. Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University. (2013).
https://search.emarefa.net/detail/BIM-346892
American Medical Association (AMA)
al-Dirdisawi, Bakr Sakha. (2013). Isolation of bioactive metabolites-producing bacteria from Ain Sarah West Karak Region. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346892
Language
English
Data Type
Arab Theses
Record ID
BIM-346892
