Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines
المؤلفون المشاركون
Mirza, Nimah
Natiq, Rakhshandeh
Soleimanjahi,Hoorieh
Amirmozafari, Nur
Riadi, Farhad
Azads, Talat Mukhtari
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 16، العدد 2 (28 فبراير/شباط 2014)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2014-02-28
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background: Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly.
Ectodomain of influenza M2 protein is a surface and highly conserved protein.
M2e in influenza vaccines may eliminate the need for changing vaccine formulation every year.
Objectives: In this study, a recombinant baculovirus containing M2e and cholera toxin subunit B fusion gene was generated with transposition process to express in large amounts in insect cell lines.
Materials and Methods: M2e-ctxB fusion gene was created and cloned into pFastBac HT.
The recombinant vector was transformed into DH10Bac cells to introduce the fusion gene into the bacmid DNA via a site-specific transposition process.
The recombinant bacmid was then extracted from white colonies and further analyzed using PCR, DNA sequence analyzing, and indirect immunofluorescence assay.
Results: PCR and DNA sequence analyzing results showed that the fusion gene was constructed as a single open reading frame and was successfully inserted into bacmid DNA.
Moreover, indirect immunofluorescence results showed that the fusion gene was successfully expressed.
Conclusions: Baculovirus expression vector system is valuable to produce M2e based influenza vaccines due to its simple utilization and ease of target gene manipulation.
The expressed protein in such systems can improve the evaluating process of new vaccination strategies
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mirza, Nimah& Natiq, Rakhshandeh& Soleimanjahi,Hoorieh& Amirmozafari, Nur& Riadi, Farhad& Azads, Talat Mukhtari. 2014. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal،Vol. 16, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-355663
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mirza, Nimah…[et al.]. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal Vol. 16, no. 2 (Feb. 2014), pp.1-7.
https://search.emarefa.net/detail/BIM-355663
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mirza, Nimah& Natiq, Rakhshandeh& Soleimanjahi,Hoorieh& Amirmozafari, Nur& Riadi, Farhad& Azads, Talat Mukhtari. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal. 2014. Vol. 16, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-355663
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-355663
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر