Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines
Joint Authors
Mirza, Nimah
Natiq, Rakhshandeh
Soleimanjahi,Hoorieh
Amirmozafari, Nur
Riadi, Farhad
Azads, Talat Mukhtari
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 16, Issue 2 (28 Feb. 2014), pp.1-7, 7 p.
Publisher
Publication Date
2014-02-28
Country of Publication
United Arab Emirates
No. of Pages
7
Main Subjects
Abstract EN
Background: Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly.
Ectodomain of influenza M2 protein is a surface and highly conserved protein.
M2e in influenza vaccines may eliminate the need for changing vaccine formulation every year.
Objectives: In this study, a recombinant baculovirus containing M2e and cholera toxin subunit B fusion gene was generated with transposition process to express in large amounts in insect cell lines.
Materials and Methods: M2e-ctxB fusion gene was created and cloned into pFastBac HT.
The recombinant vector was transformed into DH10Bac cells to introduce the fusion gene into the bacmid DNA via a site-specific transposition process.
The recombinant bacmid was then extracted from white colonies and further analyzed using PCR, DNA sequence analyzing, and indirect immunofluorescence assay.
Results: PCR and DNA sequence analyzing results showed that the fusion gene was constructed as a single open reading frame and was successfully inserted into bacmid DNA.
Moreover, indirect immunofluorescence results showed that the fusion gene was successfully expressed.
Conclusions: Baculovirus expression vector system is valuable to produce M2e based influenza vaccines due to its simple utilization and ease of target gene manipulation.
The expressed protein in such systems can improve the evaluating process of new vaccination strategies
American Psychological Association (APA)
Mirza, Nimah& Natiq, Rakhshandeh& Soleimanjahi,Hoorieh& Amirmozafari, Nur& Riadi, Farhad& Azads, Talat Mukhtari. 2014. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal،Vol. 16, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-355663
Modern Language Association (MLA)
Mirza, Nimah…[et al.]. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal Vol. 16, no. 2 (Feb. 2014), pp.1-7.
https://search.emarefa.net/detail/BIM-355663
American Medical Association (AMA)
Mirza, Nimah& Natiq, Rakhshandeh& Soleimanjahi,Hoorieh& Amirmozafari, Nur& Riadi, Farhad& Azads, Talat Mukhtari. Construction of recombinant bacmid containing M2e-Ctxb and producing the fusion protein in insect cell lines. Iranian Red Crescent Medical Journal. 2014. Vol. 16, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-355663
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6-7
Record ID
BIM-355663