Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds
المؤلفون المشاركون
Mahasini, Sawsan Hasan
al-Rimi, Rua Mahdi
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 20، العدد 2 (30 إبريل/نيسان 2013)، ص ص. 131-139، 9ص.
الناشر
تاريخ النشر
2013-04-30
دولة النشر
السعودية
عدد الصفحات
9
التخصصات الرئيسية
الموضوعات
الملخص EN
Conventional treatments for breast cancer are costly and have serious side effects.
Nonconventional natural treatments have gained wide acceptance due to their promise of a cure with minimal or no side effects, but little scientific evidence exists.
One such common remedy is the seed of the Lepidium sativum plant.
Presented here is the first reported use of the aqueous extract of Lepidium sativum seeds on breast cancer cells.
The ability of the extract to induce apoptosis and necrosis in the human breast cancer cell line MCF-7, compared to normal human skin fibroblasts (HFS), was determined by morphological changes in the cells using light microscopy, DNA fragmentation assay, and florescent stains (Annexin V and propidium iodide) using flow cytometry and fluorescent microscopy.
Apoptosis was induced in both cells, and more in MCF-7, when they were treated with 25% and 50% extract, while necrosis was observed mainly after exposure to elevated extract concentrations (75%).
DNA fragmentation resulted for both cells, in a time and dose-dependent manner.
Both cells, at all extract concentrations, showed no significant differences in the number of living, dead, apoptotic, and necrotic cells.
Finally, the results may indicate that apoptotic changes in MCF-7 may be independent of caspase-3, which is involved in apoptosis and is lacking in MCF-7 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. 2013. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences،Vol. 20, no. 2, pp.131-139.
https://search.emarefa.net/detail/BIM-357820
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences Vol. 20, no. 2 (Apr. 2013), pp.131-139.
https://search.emarefa.net/detail/BIM-357820
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences. 2013. Vol. 20, no. 2, pp.131-139.
https://search.emarefa.net/detail/BIM-357820
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 138-139
رقم السجل
BIM-357820
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر