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Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds
Joint Authors
Mahasini, Sawsan Hasan
al-Rimi, Rua Mahdi
Source
Saudi Journal of Biological Sciences
Issue
Vol. 20, Issue 2 (30 Apr. 2013), pp.131-139, 9 p.
Publisher
Publication Date
2013-04-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
Conventional treatments for breast cancer are costly and have serious side effects.
Nonconventional natural treatments have gained wide acceptance due to their promise of a cure with minimal or no side effects, but little scientific evidence exists.
One such common remedy is the seed of the Lepidium sativum plant.
Presented here is the first reported use of the aqueous extract of Lepidium sativum seeds on breast cancer cells.
The ability of the extract to induce apoptosis and necrosis in the human breast cancer cell line MCF-7, compared to normal human skin fibroblasts (HFS), was determined by morphological changes in the cells using light microscopy, DNA fragmentation assay, and florescent stains (Annexin V and propidium iodide) using flow cytometry and fluorescent microscopy.
Apoptosis was induced in both cells, and more in MCF-7, when they were treated with 25% and 50% extract, while necrosis was observed mainly after exposure to elevated extract concentrations (75%).
DNA fragmentation resulted for both cells, in a time and dose-dependent manner.
Both cells, at all extract concentrations, showed no significant differences in the number of living, dead, apoptotic, and necrotic cells.
Finally, the results may indicate that apoptotic changes in MCF-7 may be independent of caspase-3, which is involved in apoptosis and is lacking in MCF-7 cells.
American Psychological Association (APA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. 2013. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences،Vol. 20, no. 2, pp.131-139.
https://search.emarefa.net/detail/BIM-357820
Modern Language Association (MLA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences Vol. 20, no. 2 (Apr. 2013), pp.131-139.
https://search.emarefa.net/detail/BIM-357820
American Medical Association (AMA)
Mahasini, Sawsan Hasan& al-Rimi, Rua Mahdi. Apoptosis and necrosis of human breast cancer cells by an aqueous extract of garden cress (lepidium sativum) seeds. Saudi Journal of Biological Sciences. 2013. Vol. 20, no. 2, pp.131-139.
https://search.emarefa.net/detail/BIM-357820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 138-139
Record ID
BIM-357820