Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques
العناوين الأخرى
تقييم تحمل نبات البوتنيا (Petunia hybrida. L) للملوحة باستخدام تقنيات مختبرية
مقدم أطروحة جامعية
al-Attar, Fatimah Amir Abd Allah
مشرف أطروحة جامعية
Salih, Ahmad Ali
al-Mansuri, Thurayya Abd al-Rahman
الجامعة
جامعة الخليج العربي
الكلية
كلية الدراسات العليا
القسم الأكاديمي
تقنيات الاستزراع الصحراوي و الزراعة بدون تربة
دولة الجامعة
البحرين
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2012
الملخص الإنجليزي
Increased plant salt tolerance is a need in areas at risk for salinisaion.
This requires more efficient techniques for screening and selection for salt tolerance.
Although, salt stress significantly affects petunia x hybrida.
L, very little studies were done on this economically significant plant.
To understand Petunia salt tolerance mechanisms, efficient, simple and reliable in vitro methods were used in this work.
MS media fortified with 0.1mg / L BAP combined with 1 mg / L 2,4-D was used for callus induction and media and 2mg / L BA was used for shoot induction. To screen for variations in salt tolerance among Petunia genotypes, seven genotypes were investigated in vitro during seed germination, seedling, plantlets and callus growth.
The results showed that salt stress have significant effect on morphological and physiological parameters of the genotypes.
The study revealed the presence of significant differences among the genotypes in salt tolerance.
In addition, the different stages showed different responses for salt stress.
Plantlets may have the best mechanisms for salt tolerance.
Although, there are a clear differences in salt tolerance among the studied genotypes, RS genotype showed the highest salt tolerance in the Plantlets tissue organization. The selection for salt tolerance was successful in all of the stages, except seed germination where the results were not clear.
The results suggest that the salt stress could be inherited trait. The assessment of the morphological and physiological parameters showed that salt stress reduced biomass production due to ions imbalance.
Significant increase in Na+ accumulation combined with reduction in K+, Ca+ and P+ suggests nutrient deficiency that could be a factor behind the reduced FW.
This abiotic stress stimulated antioxidant enzymes (SOD and CAT) and proline accumulation combined with protein degradation.
Proline accumulation could be the best parameter for selection and screening for plant salt tolerance.
التخصصات الرئيسية
الموضوعات
عدد الصفحات
143
قائمة المحتويات
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : General materials and methods.
Chapter Four : Petunia micro propagation.
Chapter Five : Screen petunia genotypes for salt tolerance.
Chapter Six : Selection for increased salt tolerance in pink veined genotype of Petunia Hybrida L.
Chapter Seven : Conclusion and recommendations.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
al-Attar, Fatimah Amir Abd Allah. (2012). Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). Bahrain
https://search.emarefa.net/detail/BIM-357895
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
al-Attar, Fatimah Amir Abd Allah. Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). (2012).
https://search.emarefa.net/detail/BIM-357895
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
al-Attar, Fatimah Amir Abd Allah. (2012). Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). Bahrain
https://search.emarefa.net/detail/BIM-357895
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-357895
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر