Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques
Other Title(s)
تقييم تحمل نبات البوتنيا (Petunia hybrida. L) للملوحة باستخدام تقنيات مختبرية
Dissertant
al-Attar, Fatimah Amir Abd Allah
Thesis advisor
Salih, Ahmad Ali
al-Mansuri, Thurayya Abd al-Rahman
University
Arabian Gulf University
Faculty
College of Graduate Studies
Department
Desert farming Techniques and Hydroponics
University Country
Bahrain
Degree
Master
Degree Date
2012
English Abstract
Increased plant salt tolerance is a need in areas at risk for salinisaion.
This requires more efficient techniques for screening and selection for salt tolerance.
Although, salt stress significantly affects petunia x hybrida.
L, very little studies were done on this economically significant plant.
To understand Petunia salt tolerance mechanisms, efficient, simple and reliable in vitro methods were used in this work.
MS media fortified with 0.1mg / L BAP combined with 1 mg / L 2,4-D was used for callus induction and media and 2mg / L BA was used for shoot induction. To screen for variations in salt tolerance among Petunia genotypes, seven genotypes were investigated in vitro during seed germination, seedling, plantlets and callus growth.
The results showed that salt stress have significant effect on morphological and physiological parameters of the genotypes.
The study revealed the presence of significant differences among the genotypes in salt tolerance.
In addition, the different stages showed different responses for salt stress.
Plantlets may have the best mechanisms for salt tolerance.
Although, there are a clear differences in salt tolerance among the studied genotypes, RS genotype showed the highest salt tolerance in the Plantlets tissue organization. The selection for salt tolerance was successful in all of the stages, except seed germination where the results were not clear.
The results suggest that the salt stress could be inherited trait. The assessment of the morphological and physiological parameters showed that salt stress reduced biomass production due to ions imbalance.
Significant increase in Na+ accumulation combined with reduction in K+, Ca+ and P+ suggests nutrient deficiency that could be a factor behind the reduced FW.
This abiotic stress stimulated antioxidant enzymes (SOD and CAT) and proline accumulation combined with protein degradation.
Proline accumulation could be the best parameter for selection and screening for plant salt tolerance.
Main Subjects
Topics
No. of Pages
143
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : General materials and methods.
Chapter Four : Petunia micro propagation.
Chapter Five : Screen petunia genotypes for salt tolerance.
Chapter Six : Selection for increased salt tolerance in pink veined genotype of Petunia Hybrida L.
Chapter Seven : Conclusion and recommendations.
References.
American Psychological Association (APA)
al-Attar, Fatimah Amir Abd Allah. (2012). Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). Bahrain
https://search.emarefa.net/detail/BIM-357895
Modern Language Association (MLA)
al-Attar, Fatimah Amir Abd Allah. Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). (2012).
https://search.emarefa.net/detail/BIM-357895
American Medical Association (AMA)
al-Attar, Fatimah Amir Abd Allah. (2012). Evaluation of salt tolerance in Petunia hybrida. L using in vitro techniques. (Master's theses Theses and Dissertations Master). Bahrain
https://search.emarefa.net/detail/BIM-357895
Language
English
Data Type
Arab Theses
Record ID
BIM-357895
