Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma (DLBCL)‎

الملخص EN

Purpose : Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin’s lymphoma (NHL), this work was designed to study the impact of IL-10 ( 1082 G / A ; rs1800896 and 819 C / T ; rs1800871) gene promoter polymorphism on susceptibility of Egyptians to diffuse large B cell lymphoma (DLBCL) ; the major type of NHL.

To the best of our knowledge, this study is the first one that examines IL-10 promoter polymorphism in DLBCL in Egyptians.

Methods : Genotyping polymorphism is performed using sequence-specific primers polymerase chain reaction (SSP-PCR) in 100 Egyptian DLBCL patients and 119 normal controls.

Circulating plasma levels of IL-10 were measured using Enzyme-linked immunosorbent assay (ELISA).

Results : Insignificant change in IL-10 ( 1082 and 819) genotypes was recorded.

Although A allele is slightly decreased in DLBCL patients, it did not reach statistical significance.

GT haplotype was significantly elevated (P < 0.05) in NHL patients.

A significant linkage disequilibrium between the 1082 and 819 SNPs with D0 = 0.596 and r2 = 0.1032 (P < 0.001) was demonstrated.

Significantly increased plasma IL-10 (P < 0.01) was found which is positively correlated (r = 0.307 ; P < 0.01) with the disease.