Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis
مقدم أطروحة جامعية
مشرف أطروحة جامعية
al-Kamali, Hatil Hashim
Ibrahim, Hanan Muawiyah
الجامعة
جامعة أم درمان الإسلامية
الكلية
كلية العلوم و التقانة
القسم الأكاديمي
قسم التقانة الإحيائية
دولة الجامعة
السودان
الدرجة العلمية
ماجستير
تاريخ الدرجة العلمية
2012
الملخص الإنجليزي
The objectives of this study were to isolate, molecular characterize and screen of new local isolates with the potentially product agents.
Screening revealed that 35 out of 103 sample of crude cow milk (34%) were Lactococcus lactis subsp.
lactis.
Characterization of these selected isolates using conventional method, API technique and further confirmation was achieved by PCR amplifying.
All isolated exhibited marked activity against five pathogenic bacterial strains (Bacillus subtilis (ATCC), Staphylococcus aureus (ATCC 43306), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and (Clostridium perfringens), using agar well diffusion method.
Isolate 58 and 63 showed the highest activity (20mm) and (15 mm) respectively, against Gram positive bacteria B.
subtilis and S.
aureus.
Where as isolate 70 showed the highest activity (13 mm) against gram negative bacteria P.
aeruginosa, generally all aforementioned isolates were active against Gram positive and Gram negative bacteria.
Isolate 46 exhibited its maximum activity (30mm) against Clostridium perfringens at pH 2.5, isolate 42 had its highest activity (20mm) against Clostridium perfringens at temperature -4°C.
Under different UV light wave strength all isolate showed low or no activity against the different pathogenic bacteria induced in this study.
The antibacterial activity of the isolate 58 was compared with four standard commercial antibiotics ampicilin (AS), cephalexin (PR), cefotaxine (CF), cloxacillin (CX).
Showed higher activities against B.
subtilis (20mm) and (9mm) against S.
aureus more than commercial antibiotic.
In conclusion these results advocate that isolate 58 could be as a promising isolate against the Gram positive and Gram negative pathogenic bacteria with higher antibacterial activity.
التخصصات الرئيسية
الموضوعات
عدد الصفحات
98
قائمة المحتويات
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Literature reviews.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
References.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Awla, Suzan Aziz. (2012). Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-362715
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Awla, Suzan Aziz. Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University. (2012).
https://search.emarefa.net/detail/BIM-362715
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Awla, Suzan Aziz. (2012). Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-362715
لغة النص
الإنجليزية
نوع البيانات
رسائل جامعية
رقم السجل
BIM-362715
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر