Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis
Dissertant
Thesis advisor
al-Kamali, Hatil Hashim
Ibrahim, Hanan Muawiyah
University
Omdurman Islamic University
Faculty
Faculty of Sciences and Technology
Department
Department of Biotechnology
University Country
Sudan
Degree
Master
Degree Date
2012
English Abstract
The objectives of this study were to isolate, molecular characterize and screen of new local isolates with the potentially product agents.
Screening revealed that 35 out of 103 sample of crude cow milk (34%) were Lactococcus lactis subsp.
lactis.
Characterization of these selected isolates using conventional method, API technique and further confirmation was achieved by PCR amplifying.
All isolated exhibited marked activity against five pathogenic bacterial strains (Bacillus subtilis (ATCC), Staphylococcus aureus (ATCC 43306), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and (Clostridium perfringens), using agar well diffusion method.
Isolate 58 and 63 showed the highest activity (20mm) and (15 mm) respectively, against Gram positive bacteria B.
subtilis and S.
aureus.
Where as isolate 70 showed the highest activity (13 mm) against gram negative bacteria P.
aeruginosa, generally all aforementioned isolates were active against Gram positive and Gram negative bacteria.
Isolate 46 exhibited its maximum activity (30mm) against Clostridium perfringens at pH 2.5, isolate 42 had its highest activity (20mm) against Clostridium perfringens at temperature -4°C.
Under different UV light wave strength all isolate showed low or no activity against the different pathogenic bacteria induced in this study.
The antibacterial activity of the isolate 58 was compared with four standard commercial antibiotics ampicilin (AS), cephalexin (PR), cefotaxine (CF), cloxacillin (CX).
Showed higher activities against B.
subtilis (20mm) and (9mm) against S.
aureus more than commercial antibiotic.
In conclusion these results advocate that isolate 58 could be as a promising isolate against the Gram positive and Gram negative pathogenic bacteria with higher antibacterial activity.
Main Subjects
Topics
No. of Pages
98
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Literature reviews.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
Awla, Suzan Aziz. (2012). Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-362715
Modern Language Association (MLA)
Awla, Suzan Aziz. Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University. (2012).
https://search.emarefa.net/detail/BIM-362715
American Medical Association (AMA)
Awla, Suzan Aziz. (2012). Molecular characterization and antimicrobial activity of bacteriocin producing Lactococcus Lactis sub sp. Lactis. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-362715
Language
English
Data Type
Arab Theses
Record ID
BIM-362715
