Molecular and conventional identification of Trichomonas vaginalis among pregnant women attending Omdurman Maternity Hospital

مقدم أطروحة جامعية

Ali, Nazik Muhammad Hasan Muhammad

مشرف أطروحة جامعية

Umar, al-Fadil al-Ubayd

الجامعة

جامعة أم درمان الإسلامية

الكلية

كلية المختبرات الطبية

دولة الجامعة

السودان

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2011

الملخص الإنجليزي

Objective: To study the molecular characterization and frequency of Trichomonasvaginalisamong pregnant women attending Omdurman Maternity Hospital (Khartoum State). Materials and methods: High vaginal swabs were collected from three hundred pregnant women in the second trimester.

Three swabs were collected from each woman.

Swabs were investigated by wet mount microscopic examination, in pouch culture method, and real-time P.C.R.

technique.

Results: Out of 300 women investigated, 213 (71%) were found positive for T.vaginalisby microscopic examination, 148 (49.3%) were found positive by in pouch culture medium, and 267 (89%) were found positive by real-time P.C.R test.

Among the 300 pregnant women 62 (20.7%) delivered low- birth weight babies.

60 women (20%) had premature labour.

9 pregnant ladies (3.0%) had abortion.

T.

vaginaliswas predominant in the age group 21-29 years (65.3%).

148 patients were found positive by both microscopic examination and real-time P.C.R, while 87 patients were found negative by both techniques.

The sensitivity, specificity and negative predictive value of microscopic examination and real-time P.C.R.

were 79.7%, 100%, 100%, 37.9% respectively.

There was no significant difference in the results of in pouch culture and real-time P.C.R.

Frequency of abortion, occurrence of premature labour, delivery of low birth weight babies, and age groups were found significantly associated with microscopic examination results, in pouch culture results, and real-time P.C.R.

results.

Conclusion: There is an association between T.

vaginalisinfection and premature labour and delivery of low-birth weight infants.

The disease is apt to be more common in young age groups and tends to increase with pregnancy.

In pouch culture method proved to be the best method to isolate T.

vaginalisfrom vaginal discharges.

Real-time P.C.R.

technique is the highly sensitive and specific method for laboratory diagnosis of T.vaginalis.

التخصصات الرئيسية

الطب البشري

الموضوعات

• الحمل
• المرأة الحامل
• السودان

عدد الصفحات

85

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-363863