Molecular and conventional techniques for the identification of vaginal candida isolated from pregnant women attending Omdurman Maternity Hospital

مقدم أطروحة جامعية

Numayri, Habib Mirghani Abd al-Rahman

مشرف أطروحة جامعية

Umar, al-Fadil al-Ubayd

الجامعة

جامعة أم درمان الإسلامية

الكلية

كلية المختبرات الطبية

دولة الجامعة

السودان

الدرجة العلمية

دكتوراه

تاريخ الدرجة العلمية

2011

الملخص الإنجليزي

Objective To apply molecular and conventional techniques in the identification of Candida isolates among pregnant women attending Omdurman Maternity Hospital Materials and methods Two hundred eighty eight pregnant women ranging between 15-49 years of ages were randomly carried out during January 2008 to December 2010.

Demographical data were collected through an interview using structured questionnaire.Patients were examined clinically, appearance and colour of the vaginal discharge was recorded.

Three high vaginal swabs were collected from each patient one swab examined by using 10% KOH preparation and Gram stain.

The second swabs were cultured on Sabouraud dextrose agar and CHROM agar medium, and corn-meal agar medium.

The third swabs were used for molecular identification.

Candida species were identified by colonial morphology, germ tube test, and sugar utilization tests.

Antifungal sensitivity tests were done by the cup-plate agar diffusion method.

Antifungal drugs tested included clotrimazole, fluconazole, itraconazole, and nystatin.

Molecular identification was performed using the multiplex PCR technique.

Results The result revealed that frequency of vaginal candidiasis was 32.6%.

The most predominant Candida species isolated was Candia albicans(81%) followed by C.glabrata(7.4%), C.

parasilosis(6.5%) and C.

tropicalis(4.6%).

Gram stain wasfound superior (48.1%) than KOH preparation (33.7%) in the diagnosis of vaginal candidiasis.

CHROM agar medium (86.5%) was better than Sabouraud agar (62.5%) in the isolation of Candida species.

Candida species were found more sensitive (69.8%) to clotrimazole drug than nystatin (8.7%).

PCR showed a sensitivity rate of (90.4%) in the identification of Candida species.

The study showed vaginal candidiasis during pregnancy is most prevalent in the age group 20-29 years more than other group and they was relationship between prevalence of the disease and pregnancy trimesters, parity, level of education, patients occupation and husband occupation.

Conclusion This study showed that the frequency rate of vaginal candidiasis was 32.6% among pregnant women, and Candida albicanswas the commonest species isolated.

PCR technique is more sensitive than conventional methods in the diagnosis of vaginal candidiasis , so it could be used as a routine diagnosis.

The most effective antifungal drug for treatment of candidiasis was clotrimazole which was found superior than nystatin.

التخصصات الرئيسية

الطب البشري

الموضوعات

• الحمل
• المرأة الحامل
• السودان

عدد الصفحات

111

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-363945