Molecular and conventional techniques for the identification of vaginal candida isolated from pregnant women attending Omdurman Maternity Hospital
Dissertant
Numayri, Habib Mirghani Abd al-Rahman
Thesis advisor
University
Omdurman Islamic University
Faculty
Faculty of Medical Laboratory
University Country
Sudan
Degree
Ph.D.
Degree Date
2011
English Abstract
Objective To apply molecular and conventional techniques in the identification of Candida isolates among pregnant women attending Omdurman Maternity Hospital Materials and methods Two hundred eighty eight pregnant women ranging between 15-49 years of ages were randomly carried out during January 2008 to December 2010.
Demographical data were collected through an interview using structured questionnaire.Patients were examined clinically, appearance and colour of the vaginal discharge was recorded.
Three high vaginal swabs were collected from each patient one swab examined by using 10% KOH preparation and Gram stain.
The second swabs were cultured on Sabouraud dextrose agar and CHROM agar medium, and corn-meal agar medium.
The third swabs were used for molecular identification.
Candida species were identified by colonial morphology, germ tube test, and sugar utilization tests.
Antifungal sensitivity tests were done by the cup-plate agar diffusion method.
Antifungal drugs tested included clotrimazole, fluconazole, itraconazole, and nystatin.
Molecular identification was performed using the multiplex PCR technique.
Results The result revealed that frequency of vaginal candidiasis was 32.6%.
The most predominant Candida species isolated was Candia albicans(81%) followed by C.glabrata(7.4%), C.
parasilosis(6.5%) and C.
tropicalis(4.6%).
Gram stain wasfound superior (48.1%) than KOH preparation (33.7%) in the diagnosis of vaginal candidiasis.
CHROM agar medium (86.5%) was better than Sabouraud agar (62.5%) in the isolation of Candida species.
Candida species were found more sensitive (69.8%) to clotrimazole drug than nystatin (8.7%).
PCR showed a sensitivity rate of (90.4%) in the identification of Candida species.
The study showed vaginal candidiasis during pregnancy is most prevalent in the age group 20-29 years more than other group and they was relationship between prevalence of the disease and pregnancy trimesters, parity, level of education, patients occupation and husband occupation.
Conclusion This study showed that the frequency rate of vaginal candidiasis was 32.6% among pregnant women, and Candida albicanswas the commonest species isolated.
PCR technique is more sensitive than conventional methods in the diagnosis of vaginal candidiasis , so it could be used as a routine diagnosis.
The most effective antifungal drug for treatment of candidiasis was clotrimazole which was found superior than nystatin.
Main Subjects
Topics
No. of Pages
111
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction and literature review.
Chapter Two : Materials and methods.
Chapter Three : Results.
Chapter Four : Discussion and conclusion and recommendation.
References.
American Psychological Association (APA)
Numayri, Habib Mirghani Abd al-Rahman. (2011). Molecular and conventional techniques for the identification of vaginal candida isolated from pregnant women attending Omdurman Maternity Hospital. (Doctoral dissertations Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-363945
Modern Language Association (MLA)
Numayri, Habib Mirghani Abd al-Rahman. Molecular and conventional techniques for the identification of vaginal candida isolated from pregnant women attending Omdurman Maternity Hospital. (Doctoral dissertations Theses and Dissertations Master). Omdurman Islamic University. (2011).
https://search.emarefa.net/detail/BIM-363945
American Medical Association (AMA)
Numayri, Habib Mirghani Abd al-Rahman. (2011). Molecular and conventional techniques for the identification of vaginal candida isolated from pregnant women attending Omdurman Maternity Hospital. (Doctoral dissertations Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-363945
Language
English
Data Type
Arab Theses
Record ID
BIM-363945
