فصل و تشخيص بروتينات بعض أصناف التمور المحلية باستخدام كروماتوكرافي الترشيح الهلامي و الترحيل الكهربائي

الملخص EN

The present study is concerned with studying physical & chemical examinations for dates & seeds of some local varieties such as: Hallawi, Sayer & Barhee.

The results of the statistical analysis about the physical properties showed that there were high significant differences among varieties in weight, diameter, size, flesh weight & seed weight characters, but the date length did not appear any significant differences among the varieties.

T he results of the chemical composition of destoned dates showed that Barhee variety was highest in content of moisture & fat whom reached 31.43 & 0.6 % respectively & with high significant differences compare with varieties of Hallawi & Sayer, while Sayer variety showed higher content of ash which reached 2.3 %, while protein content did not show any significant differences among the varieties.

Moreover, the results of the protein extraction by different solvents for date showed that the water soluble proteins (albumins) was highest which was about 46.28-65.07 %, while was lower to salt soluble proteins (globulins) which was about 4.04-6.03 % from the total amount of proteins.

G el filtration for water soluble proteins which extracted from date showed that existence three major peaks, while salt soluble proteins showed existence two major peak with one minority peak between them, for all varieties.

In case of alcohol soluble proteins (prolamins) demonstrate existence small peak followed by one majority big peak, while alkali soluble proteins (glutenins) showed one majority big peak between two small peaks.

Electrophoresis studies showed that the salt soluble proteins extracted from date : Hallawi, Sayer & Barhee, appearance three major bands whom were molecular weights about 61000-62400 Dalton for first band, 27600-37500 Dalton for second band & 18200-23500 Dalton for third band.