Use of some antioxidant to minimize ochratoxin a toxicity in rats

الملخص EN

The present study aimed to minimize ochratoxin A toxicity by use of oral administration of two types of antioxidants, first was α-tocopherol at a concentration of 10 mg / kg body weight and the second was the extract of Hibiscus subdariffa at two concentrations 10 mg / kg and 20 mg / kg body weight, respectively.

Five groups of mature albino rats were used, group 1 was fed on a semi-synthetic feed without toxin, called control group, while group 2 was given ochratoxin A, group 3 was given ochratoxin A with a-tocopherol, group 4 and 5 was also given ochratoxin A with the extract of Hibiscus subdariffa These groups were fed on a constant semi-synthetic feed free from antioxidants, with daily oral administration of ochratoxin A and antioxidants for a period of 30 days.

The efficiency of the antioxidants were then evaluated by monitoring the changes in the serum values of some biochemical parameters.

Moreover histopathological examination of tissue specimens obtained from the kidney, liver and skeletal muscle of the rats by the use of light microscope.

The group that was treated only with ochratoxin A and without antioxidants showed elevations in the serum biochemical parameters, these were the Malonydialdehyde, glucose, triglycerides, atherogenic index (Al), liver enzymes (Aspartate Amino Transferase, Alanine Amino Transferase), creatinine and blood urea level, but caused a reduction in serum cholesterol level.

Other groups which were treated with ochratoxin A and antioxidants showed changes in the above biochemical parameters that were insignificantly differed from that of the control group.

The kidney specimens from Group 2 showed changes in proximal convoluted tubulesand the liver specimens of the same group showed involvement at the peripheral region of the hepatic lobule.

Skeletal muscle specimens showed normal architecture.

No changes were observed in all other groups investigated throughout the study regarding kidney and liver specimens, with normal anastomosing columns of hepatocytes that were having normal homogenous cytoplasm.