Antimicrobial agents from fungi : culture and partial purification

العناوين الأخرى

عوامل مضاده للميكروبات من الفطر : زراعة و تنقية جزئية

مقدم أطروحة جامعية

Hijazeen, Tehani Jazza

مشرف أطروحة جامعية

Al-Zereini, Wail A.

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

Although there is a large number and diversity of available antimicrobial drugs, new infectious diseases and resistant pathogens are emerged.

Therefore, new sources of natural bioactive products are needed to be explored.

Endophytic fungi associated with plants were found to be a vast untapped reservoir of unconventional and diverse metabolites.

Thirty nine endophytic fungal strains were isolated from different plant sources.

Twenty nine isolates were active against at least one tested bacterium in agar diffusion test.

Rhizoctonia sp.

5F was isolated from leaves of Ficus caricus plant and showed the most potent source for bioactive metabolites production.

It was cultivated in different culture media to optimize production of secondary metabolites.

The produced crude extract of this isolate was partially purified using chromatographic techniques.

The resulted fractions and sub-fractions were evaluated for their antimicrobial and antioxidant activity.

Antimicrobial activity was determined using agar diffusion and serial dilution assays.

Antioxidant capability was estimated by measuring radical scavenging ability and decolorization of DPPH.

Rhizoctonia sp.

5F produces metabolites with antibacterial activity (MIC 250 µg / ml and 15 mm inhibition zone) especially against gram positive bacteria, and compounds with antioxidant activity (IC50 161-234 µg / ml).

Endophytic microorganisms are excellent sources of bioactive natural products.

Also Rhizoctonia species have a good antibacterial activity especially against Gram positive bacteria and show good antioxidant activity.

التخصصات الرئيسية

الأحياء

الموضوعات

• الأدوية

عدد الصفحات

50

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-400985