Antimicrobial agents from fungi : culture and partial purification
Other Title(s)
عوامل مضاده للميكروبات من الفطر : زراعة و تنقية جزئية
Dissertant
Thesis advisor
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
Although there is a large number and diversity of available antimicrobial drugs, new infectious diseases and resistant pathogens are emerged.
Therefore, new sources of natural bioactive products are needed to be explored.
Endophytic fungi associated with plants were found to be a vast untapped reservoir of unconventional and diverse metabolites.
Thirty nine endophytic fungal strains were isolated from different plant sources.
Twenty nine isolates were active against at least one tested bacterium in agar diffusion test.
Rhizoctonia sp.
5F was isolated from leaves of Ficus caricus plant and showed the most potent source for bioactive metabolites production.
It was cultivated in different culture media to optimize production of secondary metabolites.
The produced crude extract of this isolate was partially purified using chromatographic techniques.
The resulted fractions and sub-fractions were evaluated for their antimicrobial and antioxidant activity.
Antimicrobial activity was determined using agar diffusion and serial dilution assays.
Antioxidant capability was estimated by measuring radical scavenging ability and decolorization of DPPH.
Rhizoctonia sp.
5F produces metabolites with antibacterial activity (MIC 250 µg / ml and 15 mm inhibition zone) especially against gram positive bacteria, and compounds with antioxidant activity (IC50 161-234 µg / ml).
Endophytic microorganisms are excellent sources of bioactive natural products.
Also Rhizoctonia species have a good antibacterial activity especially against Gram positive bacteria and show good antioxidant activity.
Main Subjects
Topics
No. of Pages
50
Table of Contents
Table of contents.
Abstract.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Design and methodology.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
Hijazeen, Tehani Jazza. (2014). Antimicrobial agents from fungi : culture and partial purification. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-400985
Modern Language Association (MLA)
Hijazeen, Tehani Jazza. Antimicrobial agents from fungi : culture and partial purification. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-400985
American Medical Association (AMA)
Hijazeen, Tehani Jazza. (2014). Antimicrobial agents from fungi : culture and partial purification. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-400985
Language
English
Data Type
Arab Theses
Record ID
BIM-400985
