Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak

العناوين الأخرى

استخلاص المواد الحيوية من البكتيريا المعزولة من التربة في منطقة الكرك-عين سارة و دراسة تأثيرها كمضادات بكتيرية

مقدم أطروحة جامعية

al-Hurani, Maysa Muhammad

مشرف أطروحة جامعية

al-Zaraini, Wail Ali Hasan
Khulayfat, Khalid Muhammad

أعضاء اللجنة

al-Shurafa, Khalid Yusuf Ahmad
al-Shakanbah, Jumah Muti
al-Harub, Amir Munawwir

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

Thirty three soil bacterial isolates were obtained from Al-Karak–Ainesarah.

The crude extract of nine strains that showed antibacterial activity were screened for their antibacterial activity against six types of pathogenic microorganisms (Bacillussubtilis, Escherichia coli, Staphylococcus aureus, Micrococcus luteus, Enterobacteraerugenes, Psedomonusaeroiginosa).

One of these isolated (D12) was shown to be the most effective strain.

Bacterial isolate named D12 was identified as Arcanobacteriumpyogenesby using RAPID CB plus kit.

Optimization of the conditions for production of bioactive extract was performed.

The crude extract of A.

pyogenes D12 has slight antibacterial activity against Micrococcus luteus whereas other gram positive and negative ones showed resistancy toward this extracts.

The crude extract of this bacterial isolate was subjected to partial purification by Thin layer chromatography.The bioactivity of the partially purified compounds was tested by agar diffusion assay and showed antimicrobial activity generally for all fractions but in similar manar to that of the crude .

التخصصات الرئيسية

الأحياء

الموضوعات

عدد الصفحات

25

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results and discussion.

Reference.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Hurani, Maysa Muhammad. (2014). Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-403664

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Hurani, Maysa Muhammad. Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-403664

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Hurani, Maysa Muhammad. (2014). Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-403664

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-403664