Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak
Other Title(s)
استخلاص المواد الحيوية من البكتيريا المعزولة من التربة في منطقة الكرك-عين سارة و دراسة تأثيرها كمضادات بكتيرية
Dissertant
Thesis advisor
al-Zaraini, Wail Ali Hasan
Khulayfat, Khalid Muhammad
Comitee Members
al-Shurafa, Khalid Yusuf Ahmad
al-Shakanbah, Jumah Muti
al-Harub, Amir Munawwir
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
Thirty three soil bacterial isolates were obtained from Al-Karak–Ainesarah.
The crude extract of nine strains that showed antibacterial activity were screened for their antibacterial activity against six types of pathogenic microorganisms (Bacillussubtilis, Escherichia coli, Staphylococcus aureus, Micrococcus luteus, Enterobacteraerugenes, Psedomonusaeroiginosa).
One of these isolated (D12) was shown to be the most effective strain.
Bacterial isolate named D12 was identified as Arcanobacteriumpyogenesby using RAPID CB plus kit.
Optimization of the conditions for production of bioactive extract was performed.
The crude extract of A.
pyogenes D12 has slight antibacterial activity against Micrococcus luteus whereas other gram positive and negative ones showed resistancy toward this extracts.
The crude extract of this bacterial isolate was subjected to partial purification by Thin layer chromatography.The bioactivity of the partially purified compounds was tested by agar diffusion assay and showed antimicrobial activity generally for all fractions but in similar manar to that of the crude .
Main Subjects
Topics
No. of Pages
25
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
Reference.
American Psychological Association (APA)
al-Hurani, Maysa Muhammad. (2014). Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-403664
Modern Language Association (MLA)
al-Hurani, Maysa Muhammad. Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-403664
American Medical Association (AMA)
al-Hurani, Maysa Muhammad. (2014). Biological characterization of active metabolites form bacteria isolated from ain Sara-Karak. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-403664
Language
English
Data Type
Arab Theses
Record ID
BIM-403664
