Isolation and characterization of proteolytic enzymes from selected plants

العناوين الأخرى

فصل و تشخيص أنزيم البروتييز من نباتات مختارة

مقدم أطروحة جامعية

Abu Murad, Mazhar Ahmad Musa

مشرف أطروحة جامعية

Atruz, Umar Muhammad Falih

الجامعة

جامعة مؤتة

الكلية

كلية العلوم

القسم الأكاديمي

قسم الأحياء

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2014

الملخص الإنجليزي

The proteolytic activity in crude extracts of pumpkin and pomegranate fruits was measured with albumin, egg albumin and casein as substrates.

The kinetic parameters (Vmax) and (Km) were investigated using the different substrates and revealed a broad specificity from both samples.

Both crude extracts appeared to be active over a wide pH range with optimum activity at pH 7 and at a temperature of 40 ˚C.

The enzymes from pumpkin were purified 6.9 fold and from pomegranate 36.5 fold.

The enzyme from both crude extracts of pumpkin and pomegranate may be a cysteine proteases as indicated by Dixon and Webb’s plot of Log10(Vmax / Km) against pH, which revealed an apparent pKa1 and pKa2 of 6.3 and 7.6, and 5.6 and 6.8, respectively, indicating that histidine and cysteine amino acid residues may be essential for enzyme activity.

التخصصات الرئيسية

الأحياء

عدد الصفحات

51

قائمة المحتويات

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Material and methods.

Chapter Four : Results and discussion.

References.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Abu Murad, Mazhar Ahmad Musa. (2014). Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-404260

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Abu Murad, Mazhar Ahmad Musa. Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-404260

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Abu Murad, Mazhar Ahmad Musa. (2014). Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-404260

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-404260