Isolation and characterization of proteolytic enzymes from selected plants
Other Title(s)
فصل و تشخيص أنزيم البروتييز من نباتات مختارة
Dissertant
Thesis advisor
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2014
English Abstract
The proteolytic activity in crude extracts of pumpkin and pomegranate fruits was measured with albumin, egg albumin and casein as substrates.
The kinetic parameters (Vmax) and (Km) were investigated using the different substrates and revealed a broad specificity from both samples.
Both crude extracts appeared to be active over a wide pH range with optimum activity at pH 7 and at a temperature of 40 ˚C.
The enzymes from pumpkin were purified 6.9 fold and from pomegranate 36.5 fold.
The enzyme from both crude extracts of pumpkin and pomegranate may be a cysteine proteases as indicated by Dixon and Webb’s plot of Log10(Vmax / Km) against pH, which revealed an apparent pKa1 and pKa2 of 6.3 and 7.6, and 5.6 and 6.8, respectively, indicating that histidine and cysteine amino acid residues may be essential for enzyme activity.
Main Subjects
No. of Pages
51
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Material and methods.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
Abu Murad, Mazhar Ahmad Musa. (2014). Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-404260
Modern Language Association (MLA)
Abu Murad, Mazhar Ahmad Musa. Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University. (2014).
https://search.emarefa.net/detail/BIM-404260
American Medical Association (AMA)
Abu Murad, Mazhar Ahmad Musa. (2014). Isolation and characterization of proteolytic enzymes from selected plants. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-404260
Language
English
Data Type
Arab Theses
Record ID
BIM-404260
