A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants

المؤلفون المشاركون

Chen, Qiang
Lai, Huafang
Brock, Christopher
He, Junyun

المصدر

Journal of Biomedicine and Biotechnology

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-12-04

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

The threat of West Nile virus (WNV) epidemics necessitates the development of a technology platform that can produce reagents to support detection and diagnosis rapidly and inexpensively.

A plant expression system is attractive for protein production due to its low-cost and high-scalability nature and its ability to make appropriate posttranslational modifications.

Here, we investigated the feasibility of using plants to produce two WNV detection and diagnostic reagents to address the current cost and scalability issues.

We demonstrated that WNV DIII antigen and E16 monoclonal antibody are rapidly produced at high levels in two plant species and are easily purified.

Furthermore, they are effective in identifying WNV and in detecting human IgM response to WNV infection.

E16 mAb does not cross-react with other flaviviruses, therefore, is valuable for improving diagnostic accuracy.

This study provides a proof of principle for using plants as a robust and economical system to produce diagnostic reagents for arboviruses.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

He, Junyun& Lai, Huafang& Brock, Christopher& Chen, Qiang. 2011. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-446899

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

He, Junyun…[et al.]. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-446899

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

He, Junyun& Lai, Huafang& Brock, Christopher& Chen, Qiang. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-446899

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-446899