A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants
Joint Authors
Chen, Qiang
Lai, Huafang
Brock, Christopher
He, Junyun
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The threat of West Nile virus (WNV) epidemics necessitates the development of a technology platform that can produce reagents to support detection and diagnosis rapidly and inexpensively.
A plant expression system is attractive for protein production due to its low-cost and high-scalability nature and its ability to make appropriate posttranslational modifications.
Here, we investigated the feasibility of using plants to produce two WNV detection and diagnostic reagents to address the current cost and scalability issues.
We demonstrated that WNV DIII antigen and E16 monoclonal antibody are rapidly produced at high levels in two plant species and are easily purified.
Furthermore, they are effective in identifying WNV and in detecting human IgM response to WNV infection.
E16 mAb does not cross-react with other flaviviruses, therefore, is valuable for improving diagnostic accuracy.
This study provides a proof of principle for using plants as a robust and economical system to produce diagnostic reagents for arboviruses.
American Psychological Association (APA)
He, Junyun& Lai, Huafang& Brock, Christopher& Chen, Qiang. 2011. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-446899
Modern Language Association (MLA)
He, Junyun…[et al.]. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-446899
American Medical Association (AMA)
He, Junyun& Lai, Huafang& Brock, Christopher& Chen, Qiang. A Novel System for Rapid and Cost-Effective Production of Detection and Diagnostic Reagents of West Nile Virus in Plants. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-446899
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446899