Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis
المؤلفون المشاركون
Ali, Imran
Lotrakul, Pongtharin
Yanwisetpakdee, Benjawan
Prasongsuk, Sehanat
Punnapayak, Hunsa
Akbar, Ali
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-14
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α-amylase in the previous studies.
In this study the α-amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column.
Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa.
The specific activity of the enzyme was found to be 131.02 U/mg.
The Lineweaver-Burk plot showed the Vmax and Km values of 8.36 U/mg and 6.33 mg/mL, respectively.
The enzyme was found to have the best activity at 5 pH, 60°C, and 30% of NaCl concentration, showing its polyextremophilic nature.
The use of various additives did not show much variation in the activity of enzyme, showing its resilience against inhibitors.
The enzyme, when tested for its use for synthetic waste water remediation by comparing its activity with commercial amylase in different salt concentrations showed that the α-amylase from A.
gracilis was having better performance at increasing salt concentrations than the commercial one.
This shows its potential to be applied in saline waste water and other low water activity effluents for bioremediation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ali, Imran& Akbar, Ali& Yanwisetpakdee, Benjawan& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. 2014. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-446911
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ali, Imran…[et al.]. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-446911
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ali, Imran& Akbar, Ali& Yanwisetpakdee, Benjawan& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-446911
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-446911
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر