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Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis
Joint Authors
Ali, Imran
Lotrakul, Pongtharin
Yanwisetpakdee, Benjawan
Prasongsuk, Sehanat
Punnapayak, Hunsa
Akbar, Ali
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α-amylase in the previous studies.
In this study the α-amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column.
Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa.
The specific activity of the enzyme was found to be 131.02 U/mg.
The Lineweaver-Burk plot showed the Vmax and Km values of 8.36 U/mg and 6.33 mg/mL, respectively.
The enzyme was found to have the best activity at 5 pH, 60°C, and 30% of NaCl concentration, showing its polyextremophilic nature.
The use of various additives did not show much variation in the activity of enzyme, showing its resilience against inhibitors.
The enzyme, when tested for its use for synthetic waste water remediation by comparing its activity with commercial amylase in different salt concentrations showed that the α-amylase from A.
gracilis was having better performance at increasing salt concentrations than the commercial one.
This shows its potential to be applied in saline waste water and other low water activity effluents for bioremediation.
American Psychological Association (APA)
Ali, Imran& Akbar, Ali& Yanwisetpakdee, Benjawan& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. 2014. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-446911
Modern Language Association (MLA)
Ali, Imran…[et al.]. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-446911
American Medical Association (AMA)
Ali, Imran& Akbar, Ali& Yanwisetpakdee, Benjawan& Prasongsuk, Sehanat& Lotrakul, Pongtharin& Punnapayak, Hunsa. Purification, Characterization, and Potential of Saline Waste Water Remediation of a Polyextremophilic α-Amylase from an Obligate Halophilic Aspergillus gracilis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-446911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446911