Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis
المؤلفون المشاركون
Alonso, Bernardo
Viale, Mariana Noelia
Montenegro, Valeria Noely
Moyano, Roberto Damián
Mon, María Laura
Duran, Rosario
Santangelo, María de la Paz
Singh, Mahavir
Gamietea, Ignacio José
Colombatti Olivieri, María Alejandra
Romano, María Isabel
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-08
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
The Delayed type hypersensitivity skin test (DTH) and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB).
The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B).
In this study, we assessed the potential of two cocktails containing M.
bovis recombinant proteins: cocktail 1 (C1): ESAT-6, CFP-10 and MPB83 and cocktail 2 (C2): ESAT-6, CFP-10, MPB83, HspX, TB10.3, and MPB70.
C1, C2, and PPD-B showed similar response by DTH in M.
bovis-sensitized guinea pigs.
Importantly, C1 induced a lower response than PPD-B in M.
avium-sensitized guinea pigs.
In cattle, C1 displayed better performance than PPD-B and C2; indeed, C1 showed the least detection of animals either vaccinated or Map-infected.
To optimize the composition of the cocktails, we obtained protein fractions from PPD-B and tested their immunogenicity in experimentally M.
bovis-infected cattle.
In one highly reactive fraction, seven proteins were identified.
The inclusion of FixB in C1 enhanced the recognition of M.
bovis-infected cattle without compromising specificity.
Our data provide a promising basis for the future development of a cocktail for TBB detection without interference by the presence of sensitized or infected animals with other mycobacteria.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mon, María Laura& Moyano, Roberto Damián& Viale, Mariana Noelia& Colombatti Olivieri, María Alejandra& Gamietea, Ignacio José& Montenegro, Valeria Noely…[et al.]. 2014. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-448982
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mon, María Laura…[et al.]. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-448982
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mon, María Laura& Moyano, Roberto Damián& Viale, Mariana Noelia& Colombatti Olivieri, María Alejandra& Gamietea, Ignacio José& Montenegro, Valeria Noely…[et al.]. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-448982
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-448982
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر