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Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis
Joint Authors
Alonso, Bernardo
Viale, Mariana Noelia
Montenegro, Valeria Noely
Moyano, Roberto Damián
Mon, María Laura
Duran, Rosario
Santangelo, María de la Paz
Singh, Mahavir
Gamietea, Ignacio José
Colombatti Olivieri, María Alejandra
Romano, María Isabel
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The Delayed type hypersensitivity skin test (DTH) and interferon-gamma assay are used for the diagnosis of bovine tuberculosis (TBB).
The specificity of these diagnoses, however, is compromised because both are based on the response against purified protein derivative of Mycobacterium bovis (PPD-B).
In this study, we assessed the potential of two cocktails containing M.
bovis recombinant proteins: cocktail 1 (C1): ESAT-6, CFP-10 and MPB83 and cocktail 2 (C2): ESAT-6, CFP-10, MPB83, HspX, TB10.3, and MPB70.
C1, C2, and PPD-B showed similar response by DTH in M.
bovis-sensitized guinea pigs.
Importantly, C1 induced a lower response than PPD-B in M.
avium-sensitized guinea pigs.
In cattle, C1 displayed better performance than PPD-B and C2; indeed, C1 showed the least detection of animals either vaccinated or Map-infected.
To optimize the composition of the cocktails, we obtained protein fractions from PPD-B and tested their immunogenicity in experimentally M.
bovis-infected cattle.
In one highly reactive fraction, seven proteins were identified.
The inclusion of FixB in C1 enhanced the recognition of M.
bovis-infected cattle without compromising specificity.
Our data provide a promising basis for the future development of a cocktail for TBB detection without interference by the presence of sensitized or infected animals with other mycobacteria.
American Psychological Association (APA)
Mon, María Laura& Moyano, Roberto Damián& Viale, Mariana Noelia& Colombatti Olivieri, María Alejandra& Gamietea, Ignacio José& Montenegro, Valeria Noely…[et al.]. 2014. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-448982
Modern Language Association (MLA)
Mon, María Laura…[et al.]. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-448982
American Medical Association (AMA)
Mon, María Laura& Moyano, Roberto Damián& Viale, Mariana Noelia& Colombatti Olivieri, María Alejandra& Gamietea, Ignacio José& Montenegro, Valeria Noely…[et al.]. Evaluation of Cocktails with Recombinant Proteins of Mycobacterium bovis for a Specific Diagnosis of Bovine Tuberculosis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-448982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448982