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Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates
المؤلفون المشاركون
Bhargavi, P.
Goyal, Dinesh
Sharma, Ashish
Jawed, M.
Goyal, Arun
Deka, Deepmoni
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-05-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The cellulase activity of Bacillus subtilis AS3 was enhanced by optimizing the medium composition by statistical methods.
The enzyme activity with unoptimised medium with carboxymethylcellulose (CMC) was 0.07 U/mL and that was significantly enhanced by CMC, peptone, and yeast extract using Placket-Burman design.
The combined effects of these nutrients on cellulase activity were studied using 22 full factorial central composite design.
The optimal levels of medium components determined were CMC (1.8%), peptone (0.8%), and yeast extract (0.479%).
The maximum enzyme activity predicted by the model was 0.49 U/mL which was in good agreement with the experimental value 0.43 U/mL showing 6-fold increase as compared to unoptimised medium.
The enzyme showed multisubstrate specificity, showing significantly higher activity with lichenan and β-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse.
The optimised medium with lichenan or β-glucan showed 2.5- or 2.8-fold higher activity, respectively, at same concentration as of CMC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deka, Deepmoni& Bhargavi, P.& Sharma, Ashish& Goyal, Dinesh& Jawed, M.& Goyal, Arun. 2011. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-449898
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deka, Deepmoni…[et al.]. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-449898
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deka, Deepmoni& Bhargavi, P.& Sharma, Ashish& Goyal, Dinesh& Jawed, M.& Goyal, Arun. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-449898
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-449898
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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