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Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates
Joint Authors
Bhargavi, P.
Goyal, Dinesh
Sharma, Ashish
Jawed, M.
Goyal, Arun
Deka, Deepmoni
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The cellulase activity of Bacillus subtilis AS3 was enhanced by optimizing the medium composition by statistical methods.
The enzyme activity with unoptimised medium with carboxymethylcellulose (CMC) was 0.07 U/mL and that was significantly enhanced by CMC, peptone, and yeast extract using Placket-Burman design.
The combined effects of these nutrients on cellulase activity were studied using 22 full factorial central composite design.
The optimal levels of medium components determined were CMC (1.8%), peptone (0.8%), and yeast extract (0.479%).
The maximum enzyme activity predicted by the model was 0.49 U/mL which was in good agreement with the experimental value 0.43 U/mL showing 6-fold increase as compared to unoptimised medium.
The enzyme showed multisubstrate specificity, showing significantly higher activity with lichenan and β-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse.
The optimised medium with lichenan or β-glucan showed 2.5- or 2.8-fold higher activity, respectively, at same concentration as of CMC.
American Psychological Association (APA)
Deka, Deepmoni& Bhargavi, P.& Sharma, Ashish& Goyal, Dinesh& Jawed, M.& Goyal, Arun. 2011. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-449898
Modern Language Association (MLA)
Deka, Deepmoni…[et al.]. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-449898
American Medical Association (AMA)
Deka, Deepmoni& Bhargavi, P.& Sharma, Ashish& Goyal, Dinesh& Jawed, M.& Goyal, Arun. Enhancement of Cellulase Activity from a New Strain of Bacillus subtilis by Medium Optimization and Analysis with Various Cellulosic Substrates. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-449898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449898