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Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis
المؤلفون المشاركون
Campos, Jacira Ribeiro
Campos, C. O.
Vireque, A. A.
Rosa-e-Silva, Ana Carolina Japur de Sá
Jamur, Maria Célia
Bernuci, M. P.
Silva-de-Sá, M. F.
المصدر
ISRN Obstetrics and Gynecology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-03
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Purpose.
To investigate whether the addition of antibiotic/antimycotic during human granulosa-lutein cells (GLCs) isolation and cell-plating procedures prevents microbial contamination after 144 h of culture and also evaluate the effects of contamination on GLCs ultrastructure and steroid secretion.
Methods.
GLCs obtained from five women submitted to assisted reproductive techniques (ARTs) were isolated with PBS supplemented with antibiotic/antimycotic or PBS nonsupplemented and cultured for 144 h.
GLCs were evaluated by transmission electron microscopy (TEM), and estradiol (E2) and progesterone (P4) secretion was assayed by chemiluminescence.
Results.
Although no contaminating microorganisms were identified by light microscopy, TEM analyses revealed several bacterial colonies in culture dishes of GLCs isolated with only PBS.
Bacterial contamination disrupted the adherence of the GLCs to the culture plate interfering with monolayer formation affecting the growth pattern of GLCs.
Various cellular debris and bacteria were observed, and no organelles were found in the cytoplasm of infected cells.
While bacterial contamination decreased estradiol media levels, it increased progesterone, as compared with noncontaminated group.
Conclusion.
Taken together, our data showed that the addition of a high dose of antibiotic/antimycotic during the isolation and cell-plating procedures prevents microbial contamination of long-term GLCs culture as its effects on cells growth and function in vitro.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Campos, C. O.& Bernuci, M. P.& Vireque, A. A.& Campos, Jacira Ribeiro& Silva-de-Sá, M. F.& Jamur, Maria Célia…[et al.]. 2012. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449991
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Campos, C. O.…[et al.]. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-449991
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Campos, C. O.& Bernuci, M. P.& Vireque, A. A.& Campos, Jacira Ribeiro& Silva-de-Sá, M. F.& Jamur, Maria Célia…[et al.]. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449991
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-449991
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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