Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis
Joint Authors
Campos, Jacira Ribeiro
Campos, C. O.
Vireque, A. A.
Rosa-e-Silva, Ana Carolina Japur de Sá
Jamur, Maria Célia
Bernuci, M. P.
Silva-de-Sá, M. F.
Source
ISRN Obstetrics and Gynecology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-03
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Purpose.
To investigate whether the addition of antibiotic/antimycotic during human granulosa-lutein cells (GLCs) isolation and cell-plating procedures prevents microbial contamination after 144 h of culture and also evaluate the effects of contamination on GLCs ultrastructure and steroid secretion.
Methods.
GLCs obtained from five women submitted to assisted reproductive techniques (ARTs) were isolated with PBS supplemented with antibiotic/antimycotic or PBS nonsupplemented and cultured for 144 h.
GLCs were evaluated by transmission electron microscopy (TEM), and estradiol (E2) and progesterone (P4) secretion was assayed by chemiluminescence.
Results.
Although no contaminating microorganisms were identified by light microscopy, TEM analyses revealed several bacterial colonies in culture dishes of GLCs isolated with only PBS.
Bacterial contamination disrupted the adherence of the GLCs to the culture plate interfering with monolayer formation affecting the growth pattern of GLCs.
Various cellular debris and bacteria were observed, and no organelles were found in the cytoplasm of infected cells.
While bacterial contamination decreased estradiol media levels, it increased progesterone, as compared with noncontaminated group.
Conclusion.
Taken together, our data showed that the addition of a high dose of antibiotic/antimycotic during the isolation and cell-plating procedures prevents microbial contamination of long-term GLCs culture as its effects on cells growth and function in vitro.
American Psychological Association (APA)
Campos, C. O.& Bernuci, M. P.& Vireque, A. A.& Campos, Jacira Ribeiro& Silva-de-Sá, M. F.& Jamur, Maria Célia…[et al.]. 2012. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449991
Modern Language Association (MLA)
Campos, C. O.…[et al.]. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-449991
American Medical Association (AMA)
Campos, C. O.& Bernuci, M. P.& Vireque, A. A.& Campos, Jacira Ribeiro& Silva-de-Sá, M. F.& Jamur, Maria Célia…[et al.]. Preventing Microbial Contamination during Long-Term In Vitro Culture of Human Granulosa-Lutein Cells : An Ultrastructural Analysis. ISRN Obstetrics and Gynecology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-449991
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449991