Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
المؤلفون المشاركون
Barros-Velázquez, Jorge
Cremonesi, P.
Calo-Mata, Pilar
Castiglioni, B.
Fernández-No, Inmaculada
Böhme, Karola
Villa, Tomás G.
Severgnini, M.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-10
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Traditional culturing methods are still commonly applied for bacterial identification in the food control sector, despite being time and labor intensive.
Microarray technologies represent an interesting alternative.
However, they require higher costs and technical expertise, making them still inappropriate for microbial routine analysis.
The present study describes the development of an efficient method for bacterial identification based on flow-through reverse dot-blot (FT-RDB) hybridization on membranes, coupled to the high specific ligation detection reaction (LDR).
First, the methodology was optimized by testing different types of ligase enzymes, labeling, and membranes.
Furthermore, specific oligonucleotide probes were designed based on the 16S rRNA gene, using the bioinformatic tool Oligonucleotide Retrieving for Molecular Applications (ORMA).
Four probes were selected and synthesized, being specific for Aeromonas spp., Pseudomonas spp., Shewanella spp., and Morganella morganii, respectively.
For the validation of the probes, 16 reference strains from type culture collections were tested by LDR and FT-RDB hybridization using universal arrays spotted onto membranes.
In conclusion, the described methodology could be applied for the rapid, accurate, and cost-effective identification of bacterial species, exhibiting special relevance in food safety and quality.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Böhme, Karola& Cremonesi, P.& Severgnini, M.& Villa, Tomás G.& Fernández-No, Inmaculada& Barros-Velázquez, Jorge…[et al.]. 2014. Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450262
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Böhme, Karola…[et al.]. Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-450262
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Böhme, Karola& Cremonesi, P.& Severgnini, M.& Villa, Tomás G.& Fernández-No, Inmaculada& Barros-Velázquez, Jorge…[et al.]. Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-450262
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-450262
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر