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Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum
المؤلفون المشاركون
Patel, Robin
Mandrekar, Jayawant N.
Cunningham, Scott A.
Rosenblatt, Jon E.
المصدر
International Journal of Bacteriology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-11
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Objective.
We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M.
hominis and Ureaplasma culture.
Methods.
283 genitourinary specimens received in the clinical bacteriology laboratory for M.
hominis and Ureaplasma species culture were evaluated.
Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0.
5 μL of the extracts were combined with 15 μL of each of the two master mixes.
Assays were performed on the LightCycler 480 II system.
Culture was performed using routine methods.
Results. M.
hominis PCR detected 38/42 M.
hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%).
Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%).
Of the specimens that tested positive for Ureaplasma species, U.
urealyticum alone was detected in 33, U.
parvum alone in 109, and both in 6.
Conclusion.
The described PCR assays are rapid alternatives to culture for detection of M.
hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U.
urealyticum from parvum.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cunningham, Scott A.& Mandrekar, Jayawant N.& Rosenblatt, Jon E.& Patel, Robin. 2013. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-451352
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cunningham, Scott A.…[et al.]. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-451352
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cunningham, Scott A.& Mandrekar, Jayawant N.& Rosenblatt, Jon E.& Patel, Robin. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-451352
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-451352
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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