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Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum
Joint Authors
Patel, Robin
Mandrekar, Jayawant N.
Cunningham, Scott A.
Rosenblatt, Jon E.
Source
International Journal of Bacteriology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M.
hominis and Ureaplasma culture.
Methods.
283 genitourinary specimens received in the clinical bacteriology laboratory for M.
hominis and Ureaplasma species culture were evaluated.
Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0.
5 μL of the extracts were combined with 15 μL of each of the two master mixes.
Assays were performed on the LightCycler 480 II system.
Culture was performed using routine methods.
Results. M.
hominis PCR detected 38/42 M.
hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%).
Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%).
Of the specimens that tested positive for Ureaplasma species, U.
urealyticum alone was detected in 33, U.
parvum alone in 109, and both in 6.
Conclusion.
The described PCR assays are rapid alternatives to culture for detection of M.
hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U.
urealyticum from parvum.
American Psychological Association (APA)
Cunningham, Scott A.& Mandrekar, Jayawant N.& Rosenblatt, Jon E.& Patel, Robin. 2013. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-451352
Modern Language Association (MLA)
Cunningham, Scott A.…[et al.]. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-451352
American Medical Association (AMA)
Cunningham, Scott A.& Mandrekar, Jayawant N.& Rosenblatt, Jon E.& Patel, Robin. Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. International Journal of Bacteriology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-451352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451352