Easy and Rapid Purification of Highly Active Nisin

الملخص EN

Nisin is an antimicrobial peptide produced and secreted by several L.

lactis strains and is specifically active against Gram-positive bacteria.

In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl.

Here, we describe an optimized purification protocol using a five-step NaCl elution to remove contaminants.

The obtained nisin is devoid of impurities and shows high bactericidal activity against the nisin-sensitive L.

lactis strain NZ9000.

Purified nisin exhibits an IC50 of ~3 nM, which is a tenfold improvement as compared to nisin obtained via the one-step elution procedure.